Susceptibility ofEscherichia coli to C2-C18 fatty acids

Abstract

The antimicrobial activity of C2–C18 fatty acids was determinedin vitro in cultures of two strains ofEscherichia coli grown on glucose. Antimicrobial activity was expressed as IC50 (a concentration at which only 50 % of the initial glucose in the cultures was utilized). Utilization of glucose was inhibited by caprylic acid (IC50 0.30–0.85 g/L) and capric acid (IC50 1.25–2.03 g/L). Neither short-chain fatty acids (C2–C6) nor fatty acids with longer chain (C12–C18) influenced substrate utilization. Caproic acid, however, decreased cell yield in cultures ofE. coli in a dose-dependent manner. No inhibition of glucose utilization was produced with unsaturated fatty acids, oleic and linoleic. Calcium ions added in excess reversed the antimicrobial effect of capric acid, but not that of caprylic acid. Antimicrobial activity of caprylic and capric acid decreased when the bacteria were grown in the presence of straw particles, or repeatedly subcultured in a medium containing these compounds at low concentrations. Counts of viable bacteria determined by plating decreased after incubation with caprylic and capric acid (30 min; 1 g/L) at pH 5.2 from >109 to ≈102/mL. A reduction of a mere 0.94–1.96 log10 CFU was observed at pH 6.5–6.6. It can be concluded that caprylic acid, and to a lesser extent also capric acid, has a significant antimicrobial activity towardE. coli. Effects of other fatty acids were not significant or absent.

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References

  1. Bearson S., Bearson B., Foster J.W.: Acid stress responses in enterobacteria.FEMS Microbiol.Lett. 147, 173–180 (1997).

    PubMed  Article  CAS  Google Scholar 

  2. Cañas-Rodriguez A., Smith H.W.: The identification of the antimicrobial factors of the stomach contents of sucking rabbits.Biochem.J. 100, 79–82 (1966).

    PubMed  Google Scholar 

  3. Cherrington C.A., Hinton M., Chopra I.: Effect of short-chain organic acids on macromolecular synthesis inEscherichia coli.J.Appl.Bacteriol. 68, 69–74 (1990).

    PubMed  CAS  Google Scholar 

  4. Clark B., Holms W.H.: Control of the sequential utilization of glucose and fructose byEscherichia coli.J.Gen.Microbiol. 95, 191–201 (1976).

    CAS  Google Scholar 

  5. Dibner J.J., Buttin P.: Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism.J.Appl. Poultry Res. 11, 453–463 (2002).

    Google Scholar 

  6. Galbraith H., Miller T.B., Paton A.M., Thompson J.K.: Antibacterial activity of long chain fatty acids and the reversal with calcium. magnesium. ergocalciferol and cholesterol.J.Appl.Bacteriol. 34, 803–813 (1971).

    PubMed  CAS  Google Scholar 

  7. Harfoot C.G., Noble R.C., Moore J.H.: The role of plant particles, bacteria and cell-free supernatant fractions of rumen contents in the hydrolysis of trilinolein and the subsequent hydrogenation of linoleic acid.Antonie van Leeuwenhoek 41, 533–542 (1975).

    PubMed  Article  CAS  Google Scholar 

  8. Hassinen J.B., Durbin G.T., Bernhart F.W.: The bacteriostatic effect of saturated fatty acids.Arch.Biochem.Biophys. 31, 183–189 (1951).

    Article  CAS  Google Scholar 

  9. Jalč D., Kišidayová S., Nerud F.: Effect of plant oils and organic acids on rumen fermentationin vitro.Folia Microbiol. 47, 171–178 (2002).

    Article  Google Scholar 

  10. Jenkins T.C., Palmquist D.L.: Effect of added fat and calcium onin vitro formation of insoluble fatty acid soaps and cell wall digestibility.J.Anim.Sci. 55, 957–963 (1982).

    CAS  Google Scholar 

  11. Lauková A., Mareková M.: Production of bacteriocins by different enterococcal isolates.Folia Microbiol. 46, 49–50 (2001).

    Article  Google Scholar 

  12. Maczulak A.E., Dehority B.A., Palmquist D.L.: Effects of long-chain fatty acids on growth of rumen bacteria.Appl.Environ.Microbiol. 42, 856–862 (1981).

    PubMed  CAS  Google Scholar 

  13. Marounek M., Skřivanová V., Savka O.G.: Effect of caprylic, capric and oleic acid on growth of rumen and rabbit cecal bacteria.J.Anim.Feed Sci. 11, 507–516 (2002).

    Google Scholar 

  14. Morovský M., Pristaš P., Javorský P.: Bacteriocins of ruminal bacteria.Folia Microbiol. 46, 61–62 (2001).

    Article  Google Scholar 

  15. Mortensen F.V., Moller J.K., Hessov I.B.: Effects of short-chain fatty acids onin vitro bacterial growth ofBacteroides fragilis andEscherichia coli.Acta Pathol.Microbiol.Immunol.Scand. 107, 240–244 (1999).

    CAS  Google Scholar 

  16. Nieman C.: Influence of trace amounts of fatty acids on the growth of microorganisms.Bacteriol.Rev. 18, 147–163 (1954).

    PubMed  CAS  Google Scholar 

  17. Pantev A., Kabadjova P., Dalgalarrondo M., Haertlé T., Ivanova I., Dousset X., Prévost H., Chobert J.-M.: Isolation and partial characterization of an antibacterial substance produced byEnterococcus faecium.Folia Microbiol. 47, 391–400 (2002).

    Article  CAS  Google Scholar 

  18. Prohászka L.: Antibacterial effect of short chain fatty acids in entericE. coli-infections of rabbits.Zbl.Vet.Med. B27, 631–639 (1980).

    Google Scholar 

  19. Ricke S.C.: Perspectives on the use of organic acids and short chain fatty acids as antimicrobials.Poultry Sci. 82, 632–639 (2003).

    CAS  Google Scholar 

  20. Sprong R.C., Hulstein M.F.E., Van der Meer R.: Bactericidal activities of milk lipids.Antimicrob.Agents Chemother. 45, 1298–1301 (2001).

    PubMed  Article  CAS  Google Scholar 

  21. Wallace R.J., Falconer M.L., Bhargava P.K.: Toxicity of short chain fatty acids at rumen pH prevents enrichment ofEscherichia coli by sorbitol in rumen contents.Curr.Microbiol. 19, 277–281 (1989).

    Article  CAS  Google Scholar 

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Correspondence to M. Marounek.

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This work was supported by grant no. QF3134 of theCzech National Agency for Agricultural Research (NAZV).

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Marounek, M., Skřivanová, E. & Rada, V. Susceptibility ofEscherichia coli to C2-C18 fatty acids. Folia Microbiol 48, 731–735 (2003). https://doi.org/10.1007/BF02931506

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Keywords

  • Antimicrobial Activity
  • Capric Acid
  • Caproic Acid
  • Viable Bacterium
  • Valeric Acid