Folia Microbiologica

, Volume 50, Issue 5, pp 457–463 | Cite as

Co-inoculation ofBorrelia afzelii with tick salivary gland extract influences distribution of immunocompetent cells in the skin and lymph nodes of mice

  • J. Severinová
  • J. Salát
  • Z. Kročová
  • J. Řezníčková
  • H. Demová
  • H. Horká
  • J. Kopecký


The impact ofIxodes ricinus salivary gland extract (SGE) on inflammatory changes in the skin and draining lymph nodes of mice, elicited by the infection with the important human pathogen,B. afzelii, was determined using flow cytometry. SGE injected together with spirochetes reduced the numbers of leukocytes and γδ-T lymphocytes in infected epidermis at early time-points post infection. In draining lymph nodes, the anti-inflammatory effect of SGE was manifested by the decrease of total cell count compared with that in mice treated with inactivated SGE. Changes in subpopulations of immunocompetent cells apparently reflected the effect of SGE on the proliferation of spirochetes in the host. The significance of tick saliva anti-inflammatory effect for saliva activated transmission ofB. afzelii is shown.


Drain Lymph Node Infected Group Borrelia Burgdorferi Immunocompetent Cell Salivary Gland Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



band pass


fluorescein-activated cell sorter


fetal calf serum


fluorescein isothiocyanate


phosphate-buffered saline


post infection


polymorphonuclear cells




saliva-activated transmission


salivary gland extract


T-cell receptor


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2005

Authors and Affiliations

  • J. Severinová
    • 1
  • J. Salát
    • 1
  • Z. Kročová
    • 2
  • J. Řezníčková
    • 1
  • H. Demová
    • 1
  • H. Horká
    • 1
  • J. Kopecký
    • 1
  1. 1.Institute of Parasitology, Academy of Sciences of the Czech Republic and Faculty of Biological SciencesUniversity of South BohemiaČeské BudějoviceCzechia
  2. 2.Institute of Radiobiology and ImmunologyPurkyně Medical AcademyHradec KrálovéCzechia

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