Abstract
Atrazine solutions (0.1, 1, 10 and 100 µg/L) inoculated withEscherichia coli andEnterococcus faecalis under natural conditions significantly increased (p ≤ 0.05) the population levels of both test bacteria; it indicates the ability of bacterial cells to degrade atrazine and to use the original compound or its degradation products as nutrient(s). In some cases, alterations in the morphology of the colonies were also observed on selective solid media. Biochemical differentiation was also found and, on the other hand, a loss of culturability was recorded; this suggests that bacteria have entered in a viable but nonculturable state. A re-appearance of the colonies occurred after inoculation on tryptone-soy agar with atrazine.
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Abbreviations
- CFU:
-
colony-forming units
- TSB:
-
tryptone-soy broth
- VBNC:
-
viable but nonculturable
References
Albanis T.A., Lambropoulou D.A., Sakkas V.A., Hela D.: Monitoring of priority pesticides using SPME (solid phase micro-extraction) in river water from Greece.Water Supply 3, 335–342 (2003).
APHA:Standard Methods for the Examination of Water and Wastewater, 18th ed. American Public Health Association, Washington (DC) 1992.
Balague C., Sturtz N., Duffard R., Evangelista de Duffard A.M.: Effect of 2,4-dichlorophenoxyacetic acid herbicide onEscherichia coli growth, chemical composition, and cellular envelope.Environ.Toxicol. 16, 43–53 (2001).
Balteanu E., Finichiu M., Schreiner M., Freund S.: Sanitary bacteriological indicators of water pollution under the action of the insecticide Dibutox (DNBF) — I. Study of quantitative and qualitative changes.J.Hyg.Epidemiol.Microbiol.Immunol. 14, 375–380 (1970).
Collier P.J., Ramsey A.J., Austin P., Gilbert P.: Growth inhibitory and biocidal activity of some isothiazolone biocides.J.Appl.Bacteriol. 69, 569–577 (1990).
Das A.C., Debnath A., Mukherjee D.: Effect of the herbicides oxadiazon and oxyfluorfen on phosphates solubilizing microorganisms and their persistence in rice fields.Chemosphere 53, 217–221 (2003).
Dutka B.J.: Coliforms are an inadequate index of water quality.J.Environ.Health 36, 39–46 (1973).
EEC:Council Directive 80/778/EEC of 15 July 1980 relating to the quality of water intended for human consumption.Offic.J.Eur.Commun. L, 229 (1980).
Finney M., Smullen J., Foster H.A., Brokx S., Storey D.M.: Evaluation of Chromocult coliform agar for the detection and enumeration ofEnterobacteriaceae from fecal samples from healthy subjects.J.Microbiol.Meth. 54, 353–358 (2003).
Foster L.J., Kwan B.H., Vancov T.: Microbial degradation of the organophosphate pesticide, Ethion.FEMS Microbiol.Lett. 240, 49–53 (2004).
Gupta S., Agrawal S.C.: Vegetative survival and reproduction under submerged and air-exposed conditions and vegetative survival as affected by salts, pesticides, and metals in aerial green algaTrentepohlia aurea.Folia Microbiol. 49, 37–40 (2004a).
Gupta S., Agrawal S.C.: Zoosporangia survival, dehiscence and zoospore formation, and motility in the green algaRhizoclonium hieroglyphicum as affected by different factors.Folia Microbiol. 49, 549–556 (2004b).
Iurovskaia E.M.: Behavior of pathogenic and sanitary indicator bacteria under conditions of reservoir pollution by organophosphate pesticides. (In Russian)Gig.Sanit. 102–103 (1975).
Karaeva N.: Effect of various herbicides on pathogenic microflora in outdoor reservoir water. (In Russian)Gig.Sanit. 24–26 (1988).
Kodama T., Ding L., Yoshida M., Yajima M.: Biodegradation of ans-triazine herbicide, simazine.J.Mol.Catal.B-Enzymol. 11, 1073–1078 (2001).
Ma J., Xu L., Wang S., Zheng R., Jin S., Huang S., Huang Y.: Toxicity of 40 herbicides to the green algaChlorella vulgaris.Ecotoxicol.Environ.Saf. 51, 128–132 (2002).
Nam I.H., Chang Y.S., Hong H.B., Lee Y.E.: A novel catabolic activity ofPseudomonas veronii in biotransformation of pentachlorophenol.Appl.Microbiol.Biotechnol. 62, 284–290 (2003).
Neidhardt S., Havemeister G., Holler C., Gundermann K.O.: Evaluation of MUG-supplemented media for the detection ofE. coli in recreational water surveillance.Zbl.Hyg.Umweltmed. 198, 152–164 (1995).
Wackett L.P., Sadowsky M.J., Martinez B., Shapir N.: Biodegradation of atrazine and relateds-triazine compounds: from enzymes to field studies.Appl.Microbiol.Biotechnol. 58, 39–45 (2002).
Yurovskaya E.M.: Behavior and indicator value of some microorganisms under conditions of soil contamination by pesticides.J.Hyg.Epidemiol.Microbiol.Immunol. 19, 10–16 (1975).
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Koutsotoli, A.D., Dimou, D.S., Alamanos, Y.P. et al. Inductive effects of environmental concentration of atrazine onEscherichia coli andEnterococcus faecalis . Folia Microbiol 50, 283–287 (2005). https://doi.org/10.1007/BF02931407
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DOI: https://doi.org/10.1007/BF02931407