Lactobacilli and enterococci — Potential probiotics for dogs

Abstract

Forty strains of enterococci and forty strains of lactobacilli isolated from feces of 10 healthy dogs were tested for the antimicrobial activity, tolerance to bile and adhesion activity. The total count of fecal enterococci reached 5.5 log CFU/g and of lactobacilli 7.6 log CFU/g. Screening for production of bacteriocin-like substances showed an to partly inhibit the growth ofEnterobacter sp. (hazy zones of inhibition). Ten strains ofEnterococcus sp. and nine strains ofLactobacillus sp. were found without any inhibitory activity against all indicators used. Seven enterococcal strains and six lactobacilli with the broadest antimicrobial spectrum were selected for further probiotic assays. In the presence of 1 % bile, the survival rate of selected enterococci (71.7–97.5 %) was higher than that of lactobacilli (66.7–75.4 %). The adhesion of strains to human intestinal mucus (5.1–8.2 % by enterococci, 2.7–8.3 % by lactobacilli) was found to be similar as adhesion to canine intestinal mucus (3.7–10.6 % by enterococci, 2.1–6.0 % by lactobacilli). Strain AD1, one lactobacillus isolate, reduced the higher level of serum cholesterol and alanine aminotransferase after oral administration to dogs suffering from diseases of the gastrointestinal tract.

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Correspondence to V. Strompfová.

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This study was supported by project VEGA 2/2043/23 ofSlovak Scientific Agency.

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Strompfová, V., Lauková, A. & Ouwehand, A.C. Lactobacilli and enterococci — Potential probiotics for dogs. Folia Microbiol 49, 203–207 (2004). https://doi.org/10.1007/BF02931403

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Keywords

  • Lactobacillus
  • Lactic Acid Bacterium
  • Lactobacillus Acidophilus
  • Adhesion Activity
  • Intestinal Mucus