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Statistical optimization of medium components for the production of xylanase byAspergillus niger KK2 in submerged cultivation

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Abstract

Medium composition was optimized for the production of xylanase byAspergillus niger KK2 using statistical experimental designs. Corn steep liquor (CSL) and industrial yeast extract (IYE) were the most important factors affecting xylanase activity. The medium that produced the optimum conditions for the production of xylanase contained 3% rice straw, 1% wheat bran, 6.3% CSL, 0.15% IYE, and 0.5% KH2PO4. After 4 days of cultivation under optimized conditions in a 2.5-L stirred tank reactor the activity and productivity of xylanase were 620 IU/mL and 6,458 IU/L.h, respectively. The highest xylanase activity obtained using the optimized medium was 80% greater than the activity obtained using basal medium. The xylanase activity predicted by a polynomial model was 670 IU/ml.

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea

    Byeong Jo Min & Chan Wha Kim

  2. CTCBIO Inc., 445-913, Hwaseong, Korea

    Yang Soon Park

  3. Department of Chemical and Biological Engineering, Korea University, 136-701, Seoul, Korea

    Seong Woo Kang, Yoon Seok Song, Jong Ho Lee & Seung Wook Kim

  4. Department of Chemical Engineering, Kwangwoon University, 139-701, Seoul, Korea

    Chulhwan Park

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  1. Byeong Jo Min
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Correspondence to Seung Wook Kim.

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Min, B.J., Park, Y.S., Kang, S.W. et al. Statistical optimization of medium components for the production of xylanase byAspergillus niger KK2 in submerged cultivation. Biotechnol. Bioprocess Eng. 12, 302–307 (2007). https://doi.org/10.1007/BF02931108

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  • DOI: https://doi.org/10.1007/BF02931108

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