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Increased hGM-CSF production and secretion with Pluronic F-68 in transgenicNicotiana tabacum suspension cell cultures

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Abstract

The effects of Pluronic F-68, a nonionic surfactant, on the production and secretion of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in a transgenicNicotiana tabacum cell suspension culture were investigated in this study. The addition of Pluronic F-68 was shown to extend cell survival in the stationary phase, but had no influence on effective initial cell growth. With regard to production, it increased the level of extracellular hGM-CSF two-fold. This may be attributable not only to the enhanced expression level, but also to the improved permeability of the cell membrane due to the interaction between Pluronic F-68 and the cell membrane and cell wall. The effect of Pluronic F-68 on the production and secretion of hGM-CSF in a bioreactor was also evaluated. hGM-CSF production in the bioreactor after the addition of Pluronic F-68 proved more effective than in flask cultures.

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Authors and Affiliations

  1. Department of Biological Engineering, Inha University, 402-751, Incheon, Korea

    Jong-Moon Cho, Jun-Young Kwon, Jung-Ae Lim & Dong-Il Kim

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  1. Jong-Moon Cho
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  2. Jun-Young Kwon
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  3. Jung-Ae Lim
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  4. Dong-Il Kim
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Correspondence to Dong-Il Kim.

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Cho, JM., Kwon, JY., Lim, JA. et al. Increased hGM-CSF production and secretion with Pluronic F-68 in transgenicNicotiana tabacum suspension cell cultures. Biotechnol. Bioprocess Eng. 12, 594–600 (2007). https://doi.org/10.1007/BF02931074

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  • DOI: https://doi.org/10.1007/BF02931074

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