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Synergic activity of selenium and probiotic bacteriumEnterococcus faecium M-74 against selected mutagens inSalmonella assay

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Abstract

Concentrated extracts of MRS (De Man-Rogosa-Sharpe) media in which probiotic bacteriumEnterococcus faecium strain M-74 was grown exerted different antimutagenic activity against ofloxacin-,N-methyl,N′-nitro-N-nitrosoguanidine- and sodium 5-nitro-2-furylacrylate-induced mutagenicity inSalmonella typhimurium assay depending on the presence (+Se) or absence of disodium selenite pentahydrate (−Se). The antimutagenicity of MRS(+Se) extract was higher than that of MRS(−Se) extract. Selenium enhanced also the antimutagenic effect of both live and killed cells ofE. faecium M-74, respectively. The live bacteria decreased the mutagenicity of selected substances more than killed cells. Synergic activity of selenium with the bacterium was also manifested.

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References

  • Annuk H., Shchepetova J., Kullisaar T., Songisepp E., Zilmer M., Mikelsaar M.: Characterization of intestinal lactobacilli as putative probiotic candidates.J.Appl.Microbiol.94, 403–412 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Ashar M.N., Prajapati J.B.: Verification of hypocholesterolemic effect of fermented milk on human subjects with different cholesterol levels.Folia Microbiol.45, 263–268 (2000).

    Article  CAS  Google Scholar 

  • Bansal M.P., Kaur T.: Growth characteristics and selenium status changes of yeast cells with inorganic and organic selenium supplementation: selenium, a chemopreventive agent.J.Med.Food5, 85–90 (2002).

    Article  PubMed  CAS  Google Scholar 

  • Belicová A., Krajčovič J., Dobias J., Ebringer L.: Antimutagenicity of milk fermented byEnterococcus faecium.Folia Microbiol.44, 513–518 (1999).

    Article  Google Scholar 

  • Benyacoub J., Czarnecki-Maulden G.L., Cavadini C., Sauthier T., Anderson R.E., Schiffrin E.J., von der Weid T.: Supplementation of food withEnterococcus faecium (SF68) stimulates immune functions in young dogs.J.Nutr.133, 1158–1162 (2003).

    PubMed  CAS  Google Scholar 

  • Cases J., Vacchina V., Napolitano A., Caporiccio B., Besancon P., Lobinski R., Rouanet J.M.: Selenium from selenium-richSpirulina is less bioavailable than selenium from sodium selenite and selenomethionine in selenium-deficient rats.J.Nutr.131, 2343–2350 (2001).

    PubMed  CAS  Google Scholar 

  • Chin-Thin W., Wei-Tun C., Tzu-Ming P., Ren-Tse W.: Blood concentrations of selenium, zinc, iron, copper and calcium in patients with hepatocellular carcinoma.Clin.Chem.Lab.Med.40, 1118–1122 (2002).

    Article  PubMed  Google Scholar 

  • Ebringer L., Ferenčik M., Lahitová N., Kačáni L., Michálková D.: Anti-mutagenic and immuno-stimulatory properties of lactic acid bacteria.World J.Microbiol.Biotechnol.11, 294–298 (1995).

    Article  Google Scholar 

  • Ebringer L., Dobias J., Krajčovič J., Polónyi J., Križková L., Lahitová N.: Antimutagens reduce ofloxacin-induced bleaching inEuglena gracilis.Mutat.Res.359, 85–93 (1996).

    PubMed  Google Scholar 

  • Ferenčík M., Ebringer L.: Modulatory effects of selenium and zinc on the immune system.Folia Microbiol.48, 417–426 (2003).

    Google Scholar 

  • Finley J.W.: Reduction of cancer risk by consumption of selenium-enriched plants: enrichment of broccoli with selenium increases the anticarcinogenic properties of broccoli.J.Med.Food6, 19–26 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Hrubý S., Špelina V., Schlemmerová A.: The significance of the consumption of specifically fermented dairy products for the composition of the intestinal microflora and for serum cholesterol values.Českoslov.Hyg.37, 109–114 (1992).

    Google Scholar 

  • Ip C., El-Bayloumy K., Upadhaya P., Ganther H., Vadhanavikit S., Thompson H.J.: Comparative effect of inorganic and organic selenocyanate derivatives in cancer chemoprevention.Carcinogenesis15, 187–192 (1994).

    Article  PubMed  CAS  Google Scholar 

  • Jacobsen C.N., Rosenfeldt., Nielsen V., Hayford A.E., Moller P.L., Michaelsen K.F., Paerregaard A., Sandstrom B., Tvede M., Jakobsen M.: Screening of probiotic activities of forty-seven strains ofLactobacillus spp. byin vitro techniques and evaluation of the colonization ability of five selected strains in humans.Appl.Environ.Microbiol.65, 4949–4956 (1999).

    PubMed  CAS  Google Scholar 

  • Jian S.W., Mei C.E., Liang Y.N., Li D., Chen Q.L., Luo H.L., Li Y.Q., Cai T.Y.: Influence of selenium-rich rice on transformation of umbilical blood B lymphocytes by Epstein-Barr virus and Epstein-Barr virus early-antigen expression.Ai Zheng22, 26–29 (2003).

    PubMed  CAS  Google Scholar 

  • Jin L.Z., Marquardt R.R., Zhao X.: A strain ofEnterococcus faecium (18C23) inhibits adhesion of enterotoxigenicEscherichia coli K88 to porcine small intestine mucus.Appl.Environ.Microbiol.66, 4200–4204 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Kaila M., Isolauri E., Soppi E., Virtanen E., Laine S., Arvilommi H.: Enhancement of the circulating antibody secreting cell response in human diarrhea by a humanLactobacillus strain.Pediatr.Res.32, 141–144 (1992).

    PubMed  CAS  Google Scholar 

  • Klein E.A., Thompson I.M., Lippman S.M., Goodman P.J., Albanes D., Taylor P.R., Coltman C.: SELECT: the selenium and vitamin E cancer prevention trial.Urol.Oncol.21, 59–65 (2003).

    PubMed  CAS  Google Scholar 

  • Kos B., Šušković J., Goreta J., Matošić S.: Effect of protectors on the viability ofLactobacillus acidophilus M92 in simulated gastrointestinal conditions.Food Technol.Biotechnol.38, 121–128 (2000).

    CAS  Google Scholar 

  • Križková L., Horniak L., Slaviková S., Ebringer L.: Protective effects of sodium selenite on foloxacin-induced loss of chloroplast DNA inEuglena gracilis.Folia Microbiol.41, 329–332 (1996).

    Article  Google Scholar 

  • Križková L., Belicova A., Dobias J., Krajčovič J., Ebringer L.: Selenium enhances the antimutagenic activity of probiotic bacteriumEnterococcus faecium M-74.World J.Microbiol.Biotechnol.18, 867–873 (2002).

    Article  Google Scholar 

  • Kullisaar T., Zilmer M., Mikelsaar M., Vihalemm T., Annuk H., Kairane C., Kilk A.: Two antioxidativelactobacilli strains as promising probiotics.Internat.J.Food Microbiol.72, 215–224 (2002).

    Article  CAS  Google Scholar 

  • Lankaputhra W.E.V., Shah N.P.: Antimutagenic properties of probiotic bacteria and of organic acids.Mutat.Res.397, 169–182 (1998).

    PubMed  CAS  Google Scholar 

  • Lauková A., Czikková S., Burdová O.: Anti-staphylococcal effect of enterocin in Sunar and yogurt.Folia Microbiol.44, 707–711 (1999).

    Article  Google Scholar 

  • Lewenstein A., Frigerio G., Moroni M.: Biological properties of SF68, a new approach for the treatment of diarrhoeal diseases.Curr.Ther.Res.26, 967–981 (1979).

    CAS  Google Scholar 

  • Li Z.Y., Guo S.Y., Li L.: Bioeffects of selenite on the growth ofSpirulina platensis and its biotransformation.Biores.Technol.89, 171–176 (2003).

    Article  CAS  Google Scholar 

  • Loguercio C., Abbiati R., Rinaldi M., Romano A., Del Vecchio Blanco C., Coltorti M.: Long-term effects ofEnterococcus faecium SF68versus lactulose in the treatment of patients with cirrhosis and grade 1–2 hepatic encephalopathy.J.Hepatol.23, 39–46 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Lopez-Saez J.B., Senra-Varela A., Pousa-Estevez L.: Selenium in breast cancer.Oncology64, 227–231 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Lu J., Pei H., Ip C., Lisk D.J., Ganther H., Thompson H.J.: Effect of an aqueous extract of selenium-enriched garlic onin vitro markers andin vivo efficacy in cancer prevention.Carcinogenesis17, 1903–1907 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Maron D.M., Ames B.N.: Revised methods for theSalmonella mutagenicity test.Mutat.Res.113, 173–215 (1983).

    PubMed  CAS  Google Scholar 

  • Mikeš Z., Ferenčik M., Jahnová E., Ebringer L., Čižnár I.: Hypocholesterolemic and immunostimulatory effects of orally appliedEnterococcus faecium M-74 in man.Folia Microbiol.40, 639–646 (1995).

    Article  Google Scholar 

  • Nadathur S.R., Gould S.J., Bakalinsky A.T.: Antimutagenicity of an acetone extract of yogurt.Mutat.Res.334, 213–224 (1995).

    PubMed  CAS  Google Scholar 

  • Olasupo N.A.: Inhibition ofListeria monocytogenes by plantaricin NA, an antibacterial substance fromLactobacillus plantarum.Folia Microbiol.43, 151–155 (1998).

    Article  CAS  Google Scholar 

  • Orrhage K., Sillerstrom E., Gustafsson J.A., Nord C.E., Rafter J.: Binding of mutagenic heterocyclic amines by intestinal and lactic acid bacteria.Mutat.Res.311, 239–248 (1994).

    PubMed  CAS  Google Scholar 

  • Osuntoki A.A., Gbenle G.O., Olukoya D.K.: Evidence for chromosomal determination of fungicidal activity in strains ofLactobacillus brevis andLactobacillus fermentum isolated from fermented foods.Folia Microbiol.48, 56–58 (2003).

    Article  CAS  Google Scholar 

  • Pantev A., Kabadjova P., Dalgalarrondo M., Haertle T., Ivanova I., Dousset N., Prevost H., Chobert J.-M.: Isolation and partial characterization of an antibacterial substance produced byEnterococcus faecium.Folia Microbiol.47, 391–400 (2002a).

    Article  CAS  Google Scholar 

  • Pantev A., Kabadjova P., Valcheva R., Danova S., Dousset X., Haertlé T., Chobert J.-M., Ivanova I.: Effects of nitrogen sources on bacteriocin production byEnterococcus faecium A-2000.Folia Microbiol.47, 659–662 (2002b).

    Article  CAS  Google Scholar 

  • Rovensky J., Šik K., Stančiková M., Ištók R., Ebringer L., Ferenčík M.: Treatment of experimental adjuvant arthritis with the combination of methotrexate and lyophilizedEnterococcus faecium enriched with organic selenium.Folia Microbiol.47, 573–578 (2002).

    Article  CAS  Google Scholar 

  • Saarela M., Mogensen G., Fonden R., Matto J., Mattila-Sandholm T.: Probiotic bacteria: safety, functional and technological properties.J.Biotechnol.84, 197–215 (2000).

    Article  PubMed  CAS  Google Scholar 

  • Salminen S., Ouwehand A.C., Benno Y., Lee Y.K.: Probiotics: how should they be defined.Trends Foods Sci.Technol.10, 107–110 (1999).

    Article  CAS  Google Scholar 

  • Schiffrin E.J., Rochat F., Link-Amster H., Aeschlimann J.M., Donnet-Hughes A.: Immunomodulation of human blood cells following the ingestion of lactic acid bacteria.J.Dairy Sci.78, 491–497 (1995).

    Article  PubMed  CAS  Google Scholar 

  • Shukla V.K., Adukia T.K., Singh S.P., Mishra C.P., Mishra R.N.: Micronutrients, antioxidants, and carcinoma of the gallbladder.J.Surg.Oncol.84, 31–35 (2003).

    Article  PubMed  CAS  Google Scholar 

  • Šuškovic J., Kos B., Matošić S., Besendorfer V.: The effect of bile salts on survival and morphology of a potential probiotic strainLactobacillus acidophilus M92.World J.Microbiol.Biotechnol.16, 673–678 (2000).

    Article  Google Scholar 

  • Vančiková Z., Lodinová-Žadníková R., Radl J., Tlaskalová-Hogenová H.: The early postnatal development of salivary antibody and immunoglobulin response in children orally colonized with a nonpathogenic, probiotic strain ofE. coli.Folia Microbiol.48, 281–288 (2003).

    Google Scholar 

  • Vogt T.M., Ziegler R.G., Graubard B.I., Swanson C.A., Greenberg R.S., Schoenberg J.B., Swanson G.M., Hayes R.B., Mayne S.T.: Serum selenium and risk of prostate cancer in US blacks and whites.Internat.J.Cancer103, 664–670 (2003).

    Article  CAS  Google Scholar 

  • Wojtczak A.: Selenium as an anticancerogenic agent.Acta Polon.Pharm.60, 215–217 (2003).

    CAS  Google Scholar 

  • Wunderlich P.F., Braun L., Fumagalli I., D’Apuzzo V., Heim F., Karly M., Lodi R., Politta G., Vonbank F., Zeltner L.: Double-blind report on the efficacy of lactic acid-producingEnterococcus SF68 in the prevention of antibiotic-associated diarrhea and in the treatment of acute diarrhea.J.Internat.Med.Res.17, 333–338 (1989).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Cell Biology, Faculty of Science, Comenius University, 811 07, Bratislava, Slovakia

    A. Belicová, L. Križková, J. Dobias, J. Krajčovič & L. Ebringer

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  1. A. Belicová
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  2. L. Križková
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  3. J. Dobias
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  4. J. Krajčovič
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  5. L. Ebringer
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Correspondence to A. Belicová.

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This research project was supported by grant fromthe Ministry of Education of the Slovak Republic, VTP 178 2000 and partially by VEGA grant no. 1/7238/20 of theSlovak Academy of Sciences.

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Belicová, A., Križková, L., Dobias, J. et al. Synergic activity of selenium and probiotic bacteriumEnterococcus faecium M-74 against selected mutagens inSalmonella assay. Folia Microbiol 49, 301–305 (2004). https://doi.org/10.1007/BF02931047

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  • DOI: https://doi.org/10.1007/BF02931047

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