Abstract
A pilot survey of sources of contamination with arcobacters (representing a potential risk for humans) was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens,etc.) from a retail level and domestic farming. Sanitary practices in slaughterhouses and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The method is based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded withArcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical of the genusArcobacter, and a product of 686 bp, specific forA. butzleri a total number of 198 samples were tested, of that 33 (17 %) were found to be positive for the genusArcobacter but only 22 (11 %) forA. butzleri.
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Vytřasová, J., Pejchalová, M., Harsová, K. et al. Isolation ofArcobacter butzleri andA. cryaerophilus in samples of meats and from meat-processing plants by a culture technique and detection by PCR. Folia Microbiol 48, 227–232 (2003). https://doi.org/10.1007/BF02930960
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DOI: https://doi.org/10.1007/BF02930960