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Physiological characterization of osmotolerant yeastPichia sorbitophila and comparison with a putative synonymPichia farinosa

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Abstract

The osmotolerant yeastPichia sorbitophila was found to differ from other yeast species, not only from the conventional ones (Saccharomyces cerevisiae, Schizosaccharomyces pombe), but also from those widely known as osmotolerant (Debaryomyces hansenii, Zygosaccharomyces rouxii).P. sorbitophila was able to survive extremely high extracellular concentrations of salts (e.g., saturated solution of KCl) and other osmolytes (70 % glucitol), although it is not classified as halophilic (or osmophilic).P. sorbitophila assimilated a broad range of carbon and nitrogen sources with extreme effectiveness. On solid media,P. sorbitophila created colonies of variable shapes and sizes in relation to media composition, number of colonies on the plate and cultivation conditions. Colonies were able to produce long-distance signals between each other that resulted in growth inhibition of the facing parts of both colonies, but were not inhibited by colonies of other yeast species growing on the same plate. Though sometimesP. sorbitophila has been indicated as a synonym ofP. farinosa, comparative physiological studies together with PCR amplification ofP. farinosa DNA fragments homologous to knownP. sorbitophila genes provided a strong indication that this strain should be classified as a separate species.

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Authors and Affiliations

  1. Institute of Physiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    L. Marešová & H. Sychrová

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  1. L. Marešová
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  2. H. Sychrová
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Correspondence to H. Sychrová.

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Marešová, L., Sychrová, H. Physiological characterization of osmotolerant yeastPichia sorbitophila and comparison with a putative synonymPichia farinosa . Folia Microbiol 48, 211–217 (2003). https://doi.org/10.1007/BF02930958

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  • DOI: https://doi.org/10.1007/BF02930958

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