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Trehalose, glycogen and ethanol metabolism in thegcrl mutant ofSaccharomyces cerevisiae

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Abstract

Since Gcr1p is pivotal in controlling the transcription of glycolytic enzymes and trehalose metabolism seems to be one of the control points of glycolysis, we examined trehalose and glycogen synthesis in response to 2 % glucose pulse during batch growth ingcr1 (glucose regulation-1) mutant lacking fully functional glycolytic pathway and in the wild-type strain. An increase in both trehalose and glycogen stores was observed 1 and 2 h after the pulse followed by a steady decrease in both the wild-type and thegcr1 mutant. The accumulation was faster while the following degradation was slower ingcr1 cells compared to wild-type ones. Although there was no distinct glucose consumption in the mutant cells it seemed that the glucose repression mechanism is similar ingcr1 mutant and in wild-type strain at least with respect to trehalose and glycogen metabolism.

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Abbreviations

Gle:

glucose

Glg:

glycogen

Tre:

trehalose

UAS:

upstream activator sequence

YP:

yeast extract-peptone

YPG:

yeast extract-peptone-glycerol

YPGal:

yeast extract-peptone-galactose

YPGL:

yeast extract-peptone-glycerol-lactate

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Author notes

  1. T. Şeker

    Present address: Center for Process Biotechnology, BioCentrum DTU, Building 223, 2800 Kgs., Lyngby, Denmark

Authors and Affiliations

  1. Department of Biotechnology, Middle East Technical University, 06 531, Ankara, Turkey

    T. Şeker & H. Hamamci

  2. Department of Food Engineering, Middle East Technical University, 06 531, Ankara, Turkey

    H. Hamamci

Authors
  1. T. Şeker
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  2. H. Hamamci
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Correspondence to T. Şeker.

Additional information

This work was supported by grant no. TOCTAG-2730 of theScientific and Technical Research Council of the Turkish Republic (TUBITAK) and by grant no. AFP-2000-07-02-12 of theInstitute of Natural and Applied Science. Middle East Technical University (Ankara).

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Şeker, T., Hamamci, H. Trehalose, glycogen and ethanol metabolism in thegcrl mutant ofSaccharomyces cerevisiae . Folia Microbiol 48, 193–198 (2003). https://doi.org/10.1007/BF02930955

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  • DOI: https://doi.org/10.1007/BF02930955

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