Abstract
The effect of isologous DNA on the course of postirradiation reparation of meristematic cells ofVicia faba primary roots was studied in detail. A considerable interest was devoted to determinations of fundamental qualitative and quantitative conditions of the above effect of isologous DNA. Main criteria of the effect were both mitotic activity of irradiated cellular population and dynamics of chromosome aberrations induced by radiation. One set of experiments compared the course of reparation as occurred in regard to applied dose of ionizing radiation in native isologous DNA, DNA denaturated by heat and degraded by DNAase, and post-irradiation reparation of induced damages was favorably affected by native isologous DNA only. Another set of results evaluated the dependence of positive reparative effect of native isologous DNA on the length of the molecule demonstrating that in the process of reparation the presence of a complete DNA macromolecule was not essential. The last experimental group was focused on observations on the dependence of the rate of native isologous DNA effect on concentration of applied solution of the macromolecule.
Abstract
Práce podrobněji studuje účinek isologní DNK na průběh postiradiační reparace meristematických buněk primárního kořeneVicia faba. Pozornost je věnována vymezení základních kvalitativních a kvantitativních podmínek uvedeného efektu isologní DNK. Jako hlavní kriteria účinku byla hodnocena jednak mitotická aktivita ozářené buněěné populace, jednak dynamika tvorby chromosomových aberací, indukovaných zářením. První serie pokusů porovnává průběh reparace v isologní DNK nativní, denaturované teplem a degradované DNasou, a to v závislosti na aplikované dávce ionizujícího zářeního. Výsledky ukázaly, že pouze isologní DNK nativní ovlivňuje příznivě postiradiační reparaci indukovaných poškození. Další serie pokusů hodnotí závislost positivního reparačního účinku nativní isologní DNK na délce její molekuly. Ukázalo se, že pro reparaci není nutná přítomnost celé makromolekuly DNK. V poslední skupině pokusů pak byla věnována pozornost závislosti míry reparačního účinku nativní isologní DNK na koncentraci aplikovaného roztoku makromolekuly.
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Šlotová, J., Karpfel, Z. & Kubíčková, D. Nativity of the macromolecule of isologous DNA as a condition for its reparative effect on radiation damage. Biol Plant 13, 100–109 (1971). https://doi.org/10.1007/BF02930753
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DOI: https://doi.org/10.1007/BF02930753