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Uptake of ammonia by saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation

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Abstract

Batch culture experiments involving ammonia uptake inSaccharomyces cerevisiae BC55 pCYG4 have been carried out. This strain carries the plasmid pCYG4 that directs substantial overproduction of NADP-GDH, conferring an 11-fold increase in activity. The wild type cells had a specific growth rate greater than BC55 pCYG4. The ammonia uptake was practically the same until 15 h of growth. However, the amount of ammonia hydroxide added during growth (60 h) was two and half times greater in the BC55 pCYG4 than wild type cells.The results suggest that the presence of the plasmid pCYG4 can increase the amount of ammonia taken by the cells, but not the amount of biomass.

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Authors and Affiliations

  1. Departamento de Bioquimica e Laboratorio de Imunopatologia,Keizo Asami, LIKA, UPFE, Cidade Uniuersitaria, CEP 50761, Recife, PE, Brazil

    J. L. Lima Filho

  2. Department of Biochemistry and Microbiology, University of St. Andrews, KYI 6 9AL, Fife, UK

    W. M. Ledingham

Authors
  1. J. L. Lima Filho
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  2. W. M. Ledingham
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Filho, J.L.L., Ledingham, W.M. Uptake of ammonia by saccharomyces cerevisiae carrying the plasmid pCYG4 related with ammonia assimilation. Appl Biochem Biotechnol 36, 107–112 (1992). https://doi.org/10.1007/BF02929690

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  • DOI: https://doi.org/10.1007/BF02929690

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