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Biochemical dehydrogenation of saccharides IV. Secondary products of biochemical dehydrogenation of D-sorbitol and D-mannitol

БИОХИМИЧЕСКАЯ ДЕГИДРОГЕНАЦИЯ САХАРИДОВ IV. Побочные продукты биохимической дегидрогенации D-со]p]бита и D-маннита

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Summary

  1. (1)

    In D-sorbitol media fermented byAcetobacter suboxydans orAcetobacter melanogenum, two reducing sugars, not. previously described, were found as side-products in addition to L-sorbose. In D-mannitol media fermented in the same way, one other reducing sugar not previously described was found in addition to D-fructose.

  2. (2)

    These secondary metabolites were isolated. From their behaviour in paper chromatography and the drop reaction it was concluded that the secondary metabolite of biochemical dehydrogenanation of sorbitol with a RF of 0.52 in development in a water-saturated phenol system was D-fructose and that the other secondary metabolite of biochemical dehydrogenation of sorbitol, which in this system had a BF of 0.29, was not identical with any of the usual sugars, but was identical with the secondary metabolite of biochemical dehydrogenation of D-mannitol.

  3. (3)

    On the basis of the conditions of formation of this other metabolite is was concluded, in agreement with previous findings in the literature, that it was 5-keto-L-sorbose (5-keto-D-fructose).

Abstract

  1. (1)

    На D-сорбитовых средах, сбраживаемых бактериями Acetobacter suboxydans или Acetobacter melanogenum, были — кроме L-сорбозы — найденыв качестве побочных продуктов еще два до сих пор не описанных восстанавливающих сахара; при таком же сбраживании D-маннита быи обнаружен — кроме D-фруктозы — еще один до сих пор не описанный восстанавливающий сахар.

  2. (2)

    Эти побочные метаболиты были выделены. Из их поведения при хроматографии на бумаге и из результатов капельных реакций вытекает, что побочным метаболитом биохимической дегидрогенации сорбита, R F котрого при проявлении в системе фенола, насыщенного водой, составляет 0,52, оказывается D-фруктоза и что второй побочный продукт биохимической дегидрогенации сорбита, обладающий в этой системе R F = 0,29, не является тожественным ни с одним из обычно встречающихся сахаров, но тожественным с пегидрогенации D-маннита.

  3. (3)

    На основе оценки условий возникновения этого второго метаболита и имеющихся до сих пор данных лит ературы было сделано заключение, что речь идет о 5-кето-L-сорбозе (5-кето-D-фруктозе).

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Authors and Affiliations

  1. Institute of Pharmacy and Biochemistry, Prague 3

    M. Kulhánek & Zdena Ševčίková

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  1. M. Kulhánek
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  2. Zdena Ševčίková
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  3. М. Кулъеанек
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  4. З. Шевчцкова
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Additional information

Papers I-III, on “Biochemical dehydrogenation of aldonic acid”, are published in Chemické listy 47 : 1071-1085, 1953.

Presented at the conference on “The utilization of the biochemistry of microorganisms in the fermentation industry”, Smolenice, May 1961

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Kulhánek, M., Ševčίková, Z., Кулъеанек, М. et al. Biochemical dehydrogenation of saccharides IV. Secondary products of biochemical dehydrogenation of D-sorbitol and D-mannitol. Folia Microbiol 7, 288–297 (1962). https://doi.org/10.1007/BF02928658

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  • DOI: https://doi.org/10.1007/BF02928658

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