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Development of bactericidal properties against gram-negative organisms in the serum of young animals

рАзвитиE БАктЕриоци дного дЕйствия нΑ грΑм-отрицΑтΕльныΕ оргΑнизмы Β сыΒороткΑх молодых животных

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Summary

The bactericidal activity of sera obtained from piglets reared without maternal colostrum and under sterile conditions, was studied. These sera, which contain complement, but not even a trace of antibody, do not have a bactericidal effect on strains ofEscherichia coli andSalmonella in the S phase.

The sera of sterile piglets artificially colonized with a given type ofEscherichia coli showed a bactericidal effect within 4—7days after administration of the bacteria. This effect was specific for the strains with which the piglets had been colonized. Bacteria of a different antigenic character were susceptible to the action of the serum of these animals only after adding a small amount of the corresponding specific antibody.

Quantitative experiments showed that the bactericidal test was extremely sensitive for determining antibodies which could be detected in concentration by three to four orders lower than by the agglutination test.

Strains not possessing a complete set of antigens (in the R phase) were susceptible no the action of the serum of newborn animals containing only complement and no antibodies. The results show that complement acts directly on the R phase of bacteria.

The view that the properdin system is identical with the bactericidal function of antibody and complement has been discussed and seems supported by these data.

Abstract

Исследовали бакте риоцидную актикность сыBороток полученных от поросят, вскормленных в ссерильных условиях, без материнского молозива. Сыворотки зтих поросят содержат комплемент, но не содержат ни малейших следов антител и не обладают бактер иоцидным действиен по отношению к микробам Ε сеса со и eаmоеа, находящимся в e-фазе

Β сыворотках стерильных молодых Животных, ис кусственно заселенных определенным типом микроба Ε. со, бактериоцидная активность была обнаружена уже на 4-7-й день после введения микробов. Зта бактериоцидная активность 0казывается спепифической только по отношению к штамму, которым поросята были заселены. СыBоротки зти поросят убивают микробы, от личающиеся по своему антигенному арактеру, т олько тогда, если

к ним прибавить в набольшом коо ичестве соот ветствующие сп ецифические антитела. Количественными опытама была уста новлена искл ючительная чу вствительностv044C; ба ктериоциднои пробы для определения антител: антитела можно обнаружить в конце нтраци0438;, которая Ha 3-5 показателей логар ифма ниже, чем для реакции агглютинации.

Антигеино неполноценно оснащенпы0435; штаммы (в -фазе) ороткой новорожденны поросят, в которой отсутствуют антитела, а имеется тол ько комплемент. езультаты опытов свидетельствуют о наличии непосредствеииого действия компле мента на бактерии в-фазе.

а основе этих экспериментальных данных в дискуссии высказывается взгляд, что пропе рдиновая система идентична с батериоцидной функцией антитела и комплемента.

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  1. Department of Immunology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 6

    J. Šterezl, J. Kostka & A. Lanc

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Šterezl, J., Kostka, J. & Lanc, A. Development of bactericidal properties against gram-negative organisms in the serum of young animals. Folia Microbiol 7, 162–174 (1962). https://doi.org/10.1007/BF02928237

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