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16α-hydroxylation of progesterone by a strain ofactinomyces globosus

16α-hydroxylation прогестерона к штамму actinomyces globosus

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Summary

In systematic study of the transformation properties of Actinomycetes it was found that 17 of 76 species tested transformed progesterone to 16α-hydroxy-progesterone. The optimal conditions for this transformation were studied in the strainActinomyces globosus, with the following results:

  1. (1)

    The optimal pH for the given type of transformation was 6–7. At lower values hydroxylation was inhibited.

  2. (2)

    Under the given experimental conditions there was no basic difference between 24-to 72-hour cultures. Older cultures were not suitable.

  3. (3)

    The course of transformation was also influenced by the steroid concentration. Under the given experimental conditions the optimal concentration was 5–10 mg. progesterone/100 ml. medium.

  4. (4)

    Isolation yielded 19.85% 16α-hy-droxyprogesterone (calculated to the initial steroid).

Abstract

В систематическое исследование трансформации свойств Actinomycetes было было установлено, что 17 из 76 видов тестировани е преобразованы прогестерон по 16 га-гидрокси -п рогестерона. Оптимальные условия для этой трансформац ии были изучены этой трансформации б ыли изучены следующие результат ы:

  1. (1)

    Оптимальное рН для данного типа трансформации была 6–7. На нижней hydroxylation ценностей была меш ает.

  2. (2)

    В соответствии с учет ом экспериментальны х условиях не было основное различие между 24–72-часовой культурами. Пожилые культурами были не пригодны.

  3. (3)

    Конечно трансформац ии была Кроме того, под влияни ем стероидных концентрации. В соответствии с учет ом экспериментальны х условиях оптимальное концентрация 5–10 мг. progesterone/100 мл. средой.

  4. (4)

    Изолирующие принесл и 19,85% 16 га-hy - droxyprogesterone (рассчитывае тся на начальном стероидные).

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Authors and Affiliations

  1. Department of Collections of Microorganisms, Institute of Biology, Czechoslovak Academy of Sciences, Prague and Institute of Pharmacy and Biochemistry, Prague

    Olga Vondrová & O. Hanč

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  1. Olga Vondrová
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  2. O. Hanč
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Vondrová, O., Hanč, O. 16α-hydroxylation of progesterone by a strain ofactinomyces globosus . Folia Microbiol 5, 247–250 (1960). https://doi.org/10.1007/BF02928156

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  • DOI: https://doi.org/10.1007/BF02928156

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