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Preliminary biochemical characteristics of the effective substance in extracts of hypersensitive cells (hypersensitivity factor) and of pyrogenic substances arising on mixing it with antigen (hypersensitivity pyrogen)

ПРЕДВАРИТЕЛЬНАЯ БИОХИМИЧЕСКАЯ ХАРАКТЕРИСТИКА ЭФФЕКТИВНОГО ВЕЩЕСТВА В ЭКСТРАКТАХ ГИПЕРСЕНСИТИВНЫХ КЛЕТОК (ГИПЕРСЕНСИТИВНЫЙ ФАКТОР) И ПИРОГЕННЫХ ВЕЩЕСТВ ВОЗНИКАУЩИХ ПОСЛЕ ЕГО СМЕШИВАНИЯ С ФНТИГЕНОМ (ГИПЕРСЕНСИТИВНЫЙ ПИРОГЕН)

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Summary

  1. (1)

    Some biochemical properties of the effective substance in extracts of cells from delayed hypersensitive animals (hypersensitivity factor) and the properties of pyrogenic substance arising in the reaction of the hypersensitivity factor with antigenin vitro (hypersensitivity pyrogen) were studied.

  2. (2)

    The hypersensitivity factor as well as the hypersensitivity pyrogen can be precipitated and fractionated under constant conditions using ammonium sulphate, ethanol in the cold and at a definite pH. Both substances are relatively heat stable and are not damaged by extraction with butanol; both substances can be prepared and kept in the freezedried state.

  3. (3)

    The results so far have shown the possibility of routine work with the hypersensitivity factor and hypersensitivity pyrogen and thus also the possibility of a biochemical approach to the solution of the problem of delayed hypersensitivity. At the same time they contributed to a further understanding of the biological nature of the reaction of delayed hypersensitive cell extract with antigenin vitro.

Abstract

  1. (1)

    Были изучены некоторые биохимические свойства эϕϕективных веществ в экстракте клеток сенсибилисированих животных (гиперсенситивный ϕактор) и свойства пирогенных веществ, возникающих при реакции гиперсенситивного ϕактора с антигеном in vitro (гиперсенситивный пироген).

  2. (2)

    Гиперсенситивный ϕактор и гиперсенситивный пироген можно осаждать и ϕракционоровать при константных условиях сернокислым аммонием, алкоголем на холоду и при определенном рН. Оба вещества относительно термосетабильны и не изменяются под влиянием экстракции бутанолом; оба вещества можно приготовить и хранить в лиоϕилизованном виде.

  3. (3)

    Имеющиеся до сих пор результаты докаъхывают возможность работы с гиперсенситивным ϕактором и гиперсерситивным пирогеном и тем самым возможность биохимического подхода крешению проблемы поздней аллергии. В то же время они способствовали дальнейшему углублению поеимания биологической сущности взаимодействия экстракта аллергических клеток с антигеном in vitro.

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Authors and Affiliations

  1. Institute of Sera and Vaccines, Prague 10

    J. Johanovský, A. Stejskal, J. Novotný, F. Škvařil & Drahomíra Francová

Authors
  1. J. Johanovský
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  2. A. Stejskal
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  3. J. Novotný
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  4. F. Škvařil
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  5. Drahomíra Francová
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Johanovský, J., Stejskal, A., Novotný, J. et al. Preliminary biochemical characteristics of the effective substance in extracts of hypersensitive cells (hypersensitivity factor) and of pyrogenic substances arising on mixing it with antigen (hypersensitivity pyrogen). Folia Microbiol 7, 331–342 (1962). https://doi.org/10.1007/BF02928121

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  • DOI: https://doi.org/10.1007/BF02928121

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