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Production of toxic antigens in dialyzed cultures of microorganisms

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Abstract

Data on the production of clostridial toxins in dialyzed cultures are summarized. Principal modifications of this cultivation technique suitable for both research and production are shown. If toxins are released from the cells by autolysis (neurotoxins ofClostridium tetani, C. botulinum; lethal factors ofC. novyi andC. sordellii), a 10-fold increase of the antigen concentration in filtrates of dialyzed cultures is found in comparison with normal cultures. If toxins are excreted already during growth (lethal factors ofC. perfringens type A, C.septicum), the positive effect of the technique is less significant. A dialyzed culture ensures a well-balanced production of toxic filtrates that contain highly concentrated, relatively pure and strongly immunogenic antigens.

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Authors and Affiliations

  1. Institute of Sera and Vaccines, 281 66, Jevany-Bohumile, Czechoslovakia

    B. Vraný & Z. Hnátková

  2. Forestry and Game Management Research Institute, 255 01, Zbraslav, Czechoslovakia

    A. Lettl

Authors
  1. B. Vraný
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  2. Z. Hnátková
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  3. A. Lettl
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Vraný, B., Hnátková, Z. & Lettl, A. Production of toxic antigens in dialyzed cultures of microorganisms. Folia Microbiol 33, 148–154 (1988). https://doi.org/10.1007/BF02928083

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  • DOI: https://doi.org/10.1007/BF02928083

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