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Cultivation on cellophane agar as a method for the assay of bacterial enzymes

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Abstract

Production of bacterial enzymes in a liquid medium and on cellophane-agar plates is compared. Using neutral proteinase, urease, α-amylase and thiosulfate sulfurtransferase as examples it was demonstrated that the cultivation on cellophane-agar plates yields higher concentrations of the enzymes and that the enzyme production is detected in a higher number of cultures. The method might be useful for the characterization of bacterial communities.

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Authors and Affiliations

  1. Institute of Sera and Vaccines, 281 66, Jevany-Bohumile, Czechoslovakia

    B. Vraný & Z. Hnátková

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  1. B. Vraný
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  2. Z. Hnátková
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Vraný, B., Hnátková, Z. Cultivation on cellophane agar as a method for the assay of bacterial enzymes. Folia Microbiol 33, 137–139 (1988). https://doi.org/10.1007/BF02928080

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  • DOI: https://doi.org/10.1007/BF02928080

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