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Heterokaryosis inPolyporus ostreiformis for improved yield of exo-1,4-α-d-glucosidase

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Abstract

An amylolytic strain ofPolyporus ostreiformis was subjected to multistep mutagenic treatment and a heterokaryotic mutant was finally selected which produced exo-1,4-α-d-glucosidase in a yield of 2.54 U/mL against the parental yield of 0.42 U/mL. The crude enzyme extract of the mutant was used to hydrolyze soluble starch, maltose and amylopectin and chromatography of the hydrolyzates revealed exo-1,4-α-D-glucosidase activity predominant in the enzyme system.

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Authors and Affiliations

  1. Department of Microbiology, Bose Institute, 700 009, Calcutta, India

    A. Pal & A. Das

  2. Department of Botany, Visva Bharati University, 731 235 West Bengal, Santiniketan, India

    A. Roy

Authors
  1. A. Pal
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  2. A. Das
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  3. A. Roy
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Pal, A., Das, A. & Roy, A. Heterokaryosis inPolyporus ostreiformis for improved yield of exo-1,4-α-d-glucosidase. Folia Microbiol 31, 203–206 (1986). https://doi.org/10.1007/BF02928001

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  • DOI: https://doi.org/10.1007/BF02928001

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