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Relationship between sporulation and synthesis of mycobacillin and dipicolinic acid under condition of catabolite repression inBacillus subtilis

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Abstract

Sporulation was repressed in the parent strain by various carbon sources whereas glucose-resistant mutants were resistant to them but not to glycerol 2-phosphate. Both mycobacillin and dipicolinic acid synthesis were repressed in the parent by some of the compounds tested,viz. glucose, pyruvate and glycerol 2-phosphate. However, these syntheses in the glucoseresistant mutants were not repressed by glucose and pyruvate but were repressed by glycerol 2-phosphate. The possible interrelationship between sporulation, dipicolinic acid and mycobacillin synthesis is discussed in light of these findings.

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References

  • Aronson A.I., Henderson E., Tincher A.: Participation of the lysine pathway in dipicolinic acid synthesis inB. cereus T.Biochem.Biophys.Res.Commun.26, 454 (1967).

    Article  PubMed  CAS  Google Scholar 

  • Banerjee A.B., Bose S.K.: Biosynthesis of mycobacillin, an antifungal peptide. IV. Effect of chloramphenicol isomers on biosynthesis.Acta Microbiol.Polon.Ser.B118, 79 (1969).

    CAS  Google Scholar 

  • Bernlohr R.W., Novblli G.D.: Some characteristics of bacitracin production byBacillus licheniformis.Arch.Biochem.Biophys.87, 232 (1960).

    Article  CAS  Google Scholar 

  • Bhattacharya P.K., Bose S.K.: Studies on sporulation and production of mycobacillin, an antifungal polypeptide, byBacillus subtilis.J.Gen.Appl.Microbiol.15, 473 (1969).

    Article  Google Scholar 

  • Church B.D., Halvorson H.: Dependence of the heat resistance of bacterial endospore on their dipicolinic acid content.Nature183, 124 (1959).

    Article  PubMed  CAS  Google Scholar 

  • Costilow R.N., Coulter W.H.: Physiological studies of an oligosporogenous strain ofBacillus popilliae.Appl. Microbiol.22, 1076 (1971).

    PubMed  CAS  Google Scholar 

  • Freese E., Klofar W., Gallier E.: Commitment to sporulation and induction of glucose — phosphoenolpyruvate-transferase.Biochim.Biophys.Acta222, 265 (1970).

    PubMed  CAS  Google Scholar 

  • Haavik H.I.: Studies on the formation of bacitracin ofBacillus licheniformis. Effect of glucose.J.Gen.Microbiol.81, 383 (1974).

    PubMed  CAS  Google Scholar 

  • Janssen F.W., Lund A.J., Anderson L.E.: Colorimetric assay for dipicolinic acid in bacterial spores.Science127, 26 (1958).

    Article  PubMed  CAS  Google Scholar 

  • Majumdar S., Das S.K., Basu S., Bose S.K.: A note on isolation of spontaneous glucose-resistant mutantof Bacillus subtilis.J.Appl.Bacteriol.56, 493 (1984).

    Google Scholar 

  • Majumdar S.K., Bose S.K.: Mycobacillin, a new antifungal antibiotic produced byBacillus subtilis.Nature181, 134 (1958).

    Article  PubMed  CAS  Google Scholar 

  • Majumdar S., Ghosh S.K., Mukhopadhyay N.K., Bose S.K.: Accumulation of peptides by mycobacillin-negative mutants ofBacillus subtilis B3.J.Gen.Microbiol. (1985).

  • Mukherjee P.K., Paulus H.: Biological function of gramicidin: Studies on gramicidin-negative mutants.Proc.Nat.Acad.Sci.USA74, 780 (1977).

    Article  PubMed  CAS  Google Scholar 

  • Ohne M., Rutberg B.: Repression of sporulation inBacillus subtilis bv L-malate.J.Bacteriol.125, 453 (1976).

    PubMed  CAS  Google Scholar 

  • Okamura S., Izaki K., Takahashi H.: Asporogenous mutants ofBacillus subtilis.J.Gen.Appl. Microbiol.16, 429 (1970).

    Article  CAS  Google Scholar 

  • Paulus H.: Polymyxins, p. 254 inAntibiotic, Vol. II (D. Gottlieb, P.D. Shaw, Eds.). Springer-Verlag, Berlin-Heidelberg-New York 1967.

    Google Scholar 

  • Pieret J.M., Demain A.L.: Sporulation and spore properties ofBacillus brevis and its gramicidins negative mutants.J.Gen.Microbiol.129, 1309 (1983).

    Google Scholar 

  • Pschorn W., Paulus H., Hansen J., Ristow H.: Induction of sporulation inBacillus brevis. 2. Dependence on the presence of the peptide antibiotics tyrocidine and linear gramicidin.Eur.J.Biochem.129, 403 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Roy B., Bose S.K.: Polypeptide antibiotic negative spore former mutants ofBacillus subtilis.J.Gen.Appl.Microbiol.17, 491 (1971).

    Article  Google Scholar 

  • Roy B., Bose S.K.: Physiology of spores of mycobacillin producer and non-producer mutants ofBacillus subtilis.Folia Microbiol.19, 203 (1974).

    Article  Google Scholar 

  • Ristow H., Pschorn W., Hansen J., Winkel U.: Induction of sporulation inBacillus brevis by peptide antibiotics.Nature280, 165 (1979).

    Article  PubMed  CAS  Google Scholar 

  • Ristow H., Paulus H.: Induction of sporulation inBacillus brevis. I. Biochemical events and modulation of RNA synthesis during induction by tyrocidine.Eur.J.Biochem.129, 395 (1982).

    Article  PubMed  CAS  Google Scholar 

  • Schaeffer P., Millet I., Aubert J.P.: Catabolite repression of bacterial sporulation.Proc. Nat.Acad.Sci.VSA54, 704 (1965).

    Article  CAS  Google Scholar 

  • Schaeffer P.: Asporogenous mutants ofBacillus subtilis Marburg.Folia Microbiol.12, 291 (1967).

    Article  CAS  Google Scholar 

  • Takahashi I.: Catabolite repression-resistant mutants ofBacillus subtilis.Can. J.Microbiol.25, 1283 (1979).

    Article  PubMed  CAS  Google Scholar 

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We thank theCouncil of Scientific and Industrial Research, India, for financial assistanc.

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Majumdar, S., Basu, S., Das, S.K. et al. Relationship between sporulation and synthesis of mycobacillin and dipicolinic acid under condition of catabolite repression inBacillus subtilis . Folia Microbiol 31, 196–202 (1986). https://doi.org/10.1007/BF02928000

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  • DOI: https://doi.org/10.1007/BF02928000

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