Abstract
DL-4-Azaleucine-resistant mutant ofBacillus licheniformis azl r- 1, isolated after N-methyl-N’-nitro-N-nitrosoguanidine mutagenesis, was a better bacitracin producer than the parent strain. In the minimal medium, the antibiotic biosynthesis was 4 times higher in the mutant than in the parent strain and less dependent on L-leucine addition. In the complex fermentation medium, the yield was 18 – 20 % higher in the mutant strain. Transaminase B activity measured in the crude extract revealed that the branched-chain amino acid biosynthetic enzymes were 5–10 times derepressed supplying bacitracin synthetase with enhanced quantity of isoleucine and leucine, the building units of bacitracin molecule.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bradford M.: A rapid and sensitive method for the quantition of microgram quantities of protein utilizing the principle of protein-dye binding.Analyt.Biochem. 72, 248 (1976).
Brenchley J.E., Williams L.S.: Transfer RNA involvement in the regulation of enzyme synthesis.Ann.Rev.Microbiol. 29, 251 (1975).
Coleman M.S., Armstrong F.B.: Branched-chain amino acid aminotransferase ofSalmonella typhimurium. I. Crystalization and preliminary characterization.Biochim.Biophys.Acta 227, 56 (1971).
Delić V., Hopwood D.A., Friend E.J.: Mutagenesis by N-methyl-N’-nitro-N-nitrosoguanidine (NTG) inStreptomyces coelicolor.Mutat.Res. 9, 167 (1970).
Drew S.W., Demain A.L.: Effect of primary metabolites on secondary metabolism.Ann.Rev. Microbiol. 31, 343 (1977).
Elander R.P., Mole J.A., Hamill R.L., Gorman M.: Biosynthesis of pyrrolnitrins by analogue- resistant mutants ofPseudomonas fluorescens.Folia Microbiol. 16, 156 (1971).
Friedberg D., Mikulka T.W., Jones J., Calvo J.M.:flrB, a regulatory locus controlling branched-chain amino acid biosynthesis inSalmonella typhimurium.J.Bacterial. 118, 942 (1974).
Frøyshov Ø., Leland S.: On the biosynthesis of bacitracin by soluble enzyme complex fromBacillus licheniformis.Eur.J.Biochem. 46, 235 (1974).
Haavik H.I., Vessia B.: Bacitracin production by the high-yielding mutantBacillus licheniformis strain AL: stimulatory effect of L-leucine.Acta Path.Microbiol.Scand.Sect. B 86, 67 (1978).
Haavik H.I.: Effects of amino acids upon bacitracin production byBacillus licheniformis.FEMS Microbiol.Lett. 10, 111 (1981).
Kline E.L., Manross D.N. Jr.,Warwick M.L.: Multivalent regulation of isoleucine —valine transaminase in anEscherichia coli K-12ilva deletion strain.J.Bacteriol. 130, 951 (1977).
Masurekar P.S., Demain A.L.: Impaired penicillin production in lysine regulatory mutants ofPenicillium chrysogenum.Antimicrob.Agents Chemother. 6, 366 (1974).
Umbarger H.E.: Regulation of amino acid biosynthesis in microorganisms, p. 1 inMTP International Review of Science, Synthesis of Amino Acids and Proteins, Vol. 7 (H.L. Kornberg, D.C. Philips, Eds). University Park Press, Baltimore 1975.
Zvenigorodsky V.l., Bugaichuk Y.D., Kuzovnikova T.A., Lukin A.A., Zhdanov V.G.: Effect of auxotrophic mutations on bacitracin production levels inBacillus licheniformis.Antibiotiki 26, 647(1981).
Author information
Authors and Affiliations
Additional information
We wish to thank Jasenka Korajlija for technical assistance.
Rights and permissions
About this article
Cite this article
Šupek, V., Gamulin, S. & Delić, V. Enhancement of bacitracin biosynthesis by branched-chain amino acids in a regulatory mutant ofBacillus licheniformis . Folia Microbiol 30, 342–348 (1985). https://doi.org/10.1007/BF02927588
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02927588