Abstract
Optimum conditions for β-galactosidase production byK. fragilis were studied. Enzyme production has a maximum after 8 –12 h of incubation. Composition of whey (from different sources) did not affect enzyme production. Different heat treatments also had no effect. Whey reconstituted to 8 –12 % total solids and adjusted to pH 4.0 afforded maximum enzyme production. Whereas inorganic nitrogen sources (specially ammonium salts) only slightly stimulated enzyme production, organic nitrogen sources (specially partially digested proteins) provided a nearly four-fold increase in enzyme production. Yeast extract and beef extract and industrial by-products like corn-steep liquor significantly stimulated enzyme production. Manganese and magnesium salts had a very little stimulation effect.
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The authors thank Dr. D. Sundaresan, Director, National Dairy Research Institute, Karnal for his interest in the work and for providing the required facilities. Two of us (H.M.S. and S.A.) gratefully acknowledge the National Dairy Research Institute and the Council of Scientific and Industrial Research for the Senior Fellowship and Junior Research Fellowship awards, respectively.
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Sonawat, H.M., Agrawal, A. & Dutta, S.M. Production of β-galactosidase fromkluyveromyces fragilis grown on whey. Folia Microbiol 26, 370–376 (1981). https://doi.org/10.1007/BF02927329
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DOI: https://doi.org/10.1007/BF02927329