Skip to main content
SpringerLink
Log in

Effect of carbon and nitrogen sources on neutral proteinase production byPseudomonas aeruginosa

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

A strain ofPseudomonas aeruginosa from soil produced large quantitaties of extracellular neutral proteinase and could utilize several organic substances as carbon and nitrogen sources for enzyme production. The growth media required the presence of a high amount of phosphate when glucose was the carbon source. The intermediates of citric-acid cycle acids supported the proteinase production more than any other carbon sources. However, complex nitrogenous substances supported enzyme production more efficiently. Higher concentration of casamino acids suppressed the proteinase synthesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chaloupka J., Křečková P.: Protease repression inBacillus megaterium KM.Biochem.Biophys.Res. Commun. 8, 120 (1962).

    Article  PubMed  CAS  Google Scholar 

  • Chaloupka J., Křečková P., Říhová L.: Independence of the formation of extracellular protease on the amino-acid level in the cells ofBacillus megaterium.Nature 200, 885 (1963).

    Article  PubMed  CAS  Google Scholar 

  • Davis R.: Microbial extracellular enzymes, their uses and some factors affecting their formation, p. 68 inBiochemistry of Industrial Microorganisms (C. Rainbow, A.H. Rose, Eds.). Academic Press, New York 1963.

    Google Scholar 

  • Fayyaz U.D., Křečková P., Chaloupka J.: Regulation of the formation of protease inBacillus megaterium. III. Enzyme production under limitation by nitrogen sources.Folia Microbiol. 14, 70 (1969).

    Article  Google Scholar 

  • Kunitz M.: Crystalline soybean trypsin inhibitors. II. General properties.J.Gen.Physiol. 30, 291 (1947).

    Article  CAS  Google Scholar 

  • Magasastik B.: Catabolite repression.Cold Spring Harbor Symp.Quant.Biol. 26, 249 (1961).

    Google Scholar 

  • Morihara K.: Studies on the protease ofPseudomonas. I. The production of the enzyme under various cultural conditions.Bull.Agr.Chem.Soc.Japan 20, 243 (1956).

    Google Scholar 

  • Stanier R.Y., Palleroni N.J., Doudoroff M.: The aerobic pseudomonads. A taxonomic study.J.Gen. Microbiol. 43, 159 (1966).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Division, Regional Research Laboratory, Jorhat-785 006, Assam, India

    J. N. Nigam, K. R. Pillai & J. N. Baruah

Authors
  1. J. N. Nigam
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. R. Pillai
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. N. Baruah
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nigam, J.N., Pillai, K.R. & Baruah, J.N. Effect of carbon and nitrogen sources on neutral proteinase production byPseudomonas aeruginosa . Folia Microbiol 26, 358–363 (1981). https://doi.org/10.1007/BF02927327

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02927327

Keywords

Search

Navigation