Summary
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(1)
The increase in the resistance of speculating cells ofBacillus cereus to X-rays commences during the prespore phase, when the proteins of the sporangium are being enriched with cystine. The possibility that the cystine structure contributes to the radioresistance of the spores is discussed.
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(2)
The relative increase in radio-resistance occurs before the synthesis of dipicolinic acid and the incorporation of45calcium, but continues to increase gradually in the course of further development.
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(3)
The resistance of sporulating cells to higher temperatures develops progressively, according to the amount of dipicolinic acid synthesized.
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(1)
Начало повышения устойчивости спорообразующих клеток Bacillus cereus к лучам Х относитя к периоду образования преспор, когда протеины спорангиев обогащаются цистином. — Обсуждается возможность участия цистиновой структуры в радиорезистентност и спор.
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(2)
Относительное повышение радиорезистентност инаблюдается уже перед синтезомдипиколино вой кислоты и включением Са45, но в течение дальнейшего развития радиорезистентност ь продолжает постепенно повышаться.
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(3)
Устойчивость спорообразующих клеток к повышенным температурам развивается постепенно в зависимости от количества синтезируемой дипиколиновой кислоты.
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References
Alexander, P., Charlesby, A.:Energy transfer in macromolecules exposed to ionizing radiations. Nature 173: 578, 1954.
Black, S. H., Hashimoto, T., Gerhardt, P.:Calcium reversal of the heat susceptibility and dipicolinate deficiency of spores formed “endotrophically” in water. Can. J. Microbiol. 6: 213, 1960.
Braams, R.:Changes in the radiation sensitivity of some enzymes and the possibility of protection against the direct action of ionizing particles. Radiation Research 12: 113, 1960.
Cavallini, D., Mondori, B., Giovanella, B., De Marco, C.:Disulfide interchange by ionizing radiation. Science 131: 1441, 1960.
Elujarn, L., Pihl, A.:On the mode of action of X-ray protective agents. II. Interaction between biologically important thiols and disulfides. J. biol. Chem. 225: 499, 1957.
Eldjarn, L., Pihl, A.:The cysteine-cysteamine group of protective agents: chemical structure, protective ability and mixed disulfide formation. Radiation Research 9: 110, 1958.
Gordy, W., Ard, W. B., Shields, H.:Microwave spectroscopy of biological substances. I. Paramagnetic resonance in X-irradiated ami to acids and proteins. Proc. nat. Acad. Sci. 41: 983, 1955.
Gordy, W., Miyagawa, I.:Electron spin resonance studies of mechanisms for chemical protection from ionizing radiation. Radiation Research 12: 211, 1960.
Gordy, W., Shields, H.:Electron spin resonance studies of radiation damage to proteins. Radiation Research 9: 611, 1958.
Hashimoto, T., Black, S. H., Gerhardt, P.:Development of fine structure, thermostability and dipicolinate during sporogenesia in a bacillus Can. J. Microbiol. 6: 203, 1960.
Jansoen, F. W., Lund, A. J., Anderson, L. E.:Colorimetric assay for dipicolinic acid in bacterial spores. Science 127: 26, 1958.
Jones Mark, M.:An alternative mechanism for chemical protection against radiation damage. Nature 185: 96, 1960.
Leif, W. R., Herbert, J. E.:Effect of urocanic acid and dipicolinic acid on bacteria exposed to ultraviolet radiation. Amer. J. Hyg. 71: 285, 1960.
Norman, A., Ginoza, W.:Molecular interactions in irradiated solids. Radiation Research 9: 77, 1958.
Ray, D. K., Hutchinson, F., Morowitz, H. J.:A connexion between S-S bond breakage and inactivation by radiation of a dry enzyme. Nature 186: 312, 1960.
Romig, W. R., Wyss, O.:Some effects of ultraviolet radiation on sporulating cultures of Bacillus cereus. J. Bacteriol. 74: 386, 1957.
Shinohara, K.:The determination of thiol and disulfide compounds, with special reference to cysteine and cystine IV. A precision method for the determination of thiol compounds applied to the standardization of cysteine hydrochloride. J. biol. Chem. 112: 671, 1936.
Smaller, B., Avery, E. C.:Radiation protection and free radicals. Nature 183: 539, 1959.
Vinter, V.:Inequality of the cells of Bacillus megathe rium during sporogenesis. Fol. biol. (Praha) 3: 188, 1955.
Vinter, V.:Sporulation of bacilli. Consumption of calcium by the cells and decrease in the proteolytic activity of the medium during sporulation of Bacillus megatherium. Fol. biol. (Praha) 4: 216, 1956.
Vinter, V.:Differences in cysl(e)ine content between vegetative cells and spores of Bacillus cereus and Bacillus megatherium. Nature 183: 998, 1959a.
Vinter, V.:Sporulation of bacilli VII. The participation of cysteine and cystine in spore formation by Bacillus megatherium. Fol. microbiol. 4: 216, 1959b.
Vinter, V.:Spores of microorganisms VIII. The synthesis of specific calcium- and cystine-containing structures in sporulating cells of bacilli. Fol. microbiol. 5: 217, 1960a.
Vinter, V.:The formation of cystine-rich structure in sporulating cells and its possible role in the resistance of spores. Second Conference on Spores, University of Illinois, Urbana, USA, 1960b.
Vinter, V.:The changes in radioresistance of sporulating cells of Bacillus cereus. Nature 189: 589, 1961.
Woese, C:The effect of ionizing and ultraviolet radiation on germi lating cultures of B. megatherium spores. Abstr. Biophys. Soo. Meeting, p. 42, 1958.
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Vinter, V. Spores of microorganisms. Folia Microbiol 7, 115–120 (1962). https://doi.org/10.1007/BF02927234
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DOI: https://doi.org/10.1007/BF02927234