Abstract
Batch cultures ofA. vinelandii in ammonium phosphate-limited and N-free phosphate-limited media were compared with control cultures (N-free phosphate-sufficient media). The effects of phosphate limitation on growth were determined by viable cells counts. Under phosphate-limitation conditions, growth inhibition and decreased viability were observed. Intracellular levels of RNA, poly-3-hydroxybutyrate, phosphate and oxygen uptake were significantly affected by phosphate limitation. When phosphate-limited cultures were examined microscopically, pleomorphism was more marked than in control cultures. Also phosphate-limited cells showed an increase in resistance to UV irradiation, mechanical disruption, desiceation and the combined action of ethylenediaminetetraacetie acid and lysozyme.
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Martinez-Toledo, M.V., Gonzalez-Lopez, J., Salmeron, V. et al. Properties ofAzotobacter vinelandii in phosphate-limited batch cultures. Folia Microbiol 31, 154–163 (1986). https://doi.org/10.1007/BF02926834
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DOI: https://doi.org/10.1007/BF02926834