Skip to main content
Log in

Properties of cellulase fromTrichoderma viride

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Cellulase produced byTrichoderma viride acted on carboxymethyl cellulose with a Km of 4.9 g substrate per litre, showing a pH optimum at 4.5 and a temperature optimum at 55 °C. Ag+, Hg2+, Zn2+, Cu2+ and N3 - were inhibitory.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Betrabet S. M., Patel U. T.: Cellulolytic microorganisms II. Cellulolytic enzymes of Streptomyøes andAspergillua terreus.Labdev. Part B 7, 221 (1969).

    CAS  Google Scholar 

  • Chang W. S., Usami S.: Cellulase VII. Salting out and some properties of cellulolytic enzyme fromTrichoderma viride.Hakko Kypkaisi 26, 73 (1968).

    CAS  Google Scholar 

  • Gupta J. K., Das N. B., Gupta Y. P.: Effect of cultural conditions on cellulase formation byTrichoderma viride.Agr. Biol. Chem, (Tokyo) 36, 1961 (1972).

    CAS  Google Scholar 

  • Gupta J. K., Gupta Y. P., Das N. B.: Degradation of cellulosic materials byTrichoderma viride cellulase.Agr. Biol. Chem. (Tokyo) 37, 2657 (1973).

    CAS  Google Scholar 

  • Iwasaki Y., Niihara T., Matsuda T., Hiromi I.: Cellulase.Nippon Nogyo Kankyusho Nempo 23, 43 (1966).

    Google Scholar 

  • Jothianadan D., Shanmugasundaram E. R. B.: Cellulase ofPiricularia oryzae.Enzymologia 35, 11 (1968).

    Google Scholar 

  • Keilich G., Bailey P. G., Afting E. G., Liese W.: Cellulase from the wood degrading fungusPolyporus schweinitzii.Biochim. Biophys. Acta 185, 392 (1969).

    PubMed  CAS  Google Scholar 

  • Kooiman P., Roclofren P. A., Sweeris S.: Some properties of cellulase fromMyrothecium verrucaria.Enzymologia 16, 237 (1953–54).

    PubMed  CAS  Google Scholar 

  • Kunihisa G., Chang W., Usami S.: Cellulases. X. Assay of carboxymethyl cellulase activity and enzyme mechanism of hydrolysis of CMC.Hako Kyokaishi 27, 95 (1969).

    Google Scholar 

  • Kurodo H., Mochizuhi I.: Cellulolytic enzyme produced byNeurospora sp. I. Culture conditions and some properties of the crude enzyme.Hokko Kogaku Zasshi 45 330 (1967).

    Google Scholar 

  • Lowry O. H. Rosebrough N. J., Fake A. L., Randall R. J.: Protein measurement with the Folinphenol reagent.J. Biol. Chem. 193 265 (1951).

    PubMed  CAS  Google Scholar 

  • Miller G. L.: Use of dinitrosalicylic acid reagent for determination of reducing sugars.Analyt. Chem. 31, 426 (1963).

    Article  Google Scholar 

  • Reese E. T., Mandels M.: Enzymatic hydrolysis of cellulase and its derivatives.Meth. Carbogydrate Chem. 3, 139 (1963).

    CAS  Google Scholar 

  • Shigetaka I., Kikuchi T., Yokotsuka T.: Enzymic degradation of plant tissue. I. Degradation of enzyme of plant tissue and general nature of cellulolytic enzymes produced byAspergillus sojae No. 48.Nippon Nogei Kogaku Kaishi 43, 536 (1969).

    Google Scholar 

  • Singh P. G.: Extracellular hydrolytic enzymes ofFusarium lateritium.Indian J. Microbiol. 8, 95 (1968).

    CAS  Google Scholar 

  • Sison B. C., Schubert W. J., Nord F. F.: On the mechanism of enzyme action. LXV. A cellulolytic enzyme from the moldPoria vailantii.Arch. Biochem. Biophys. 75, 260 (1958).

    Article  PubMed  CAS  Google Scholar 

  • Sumber J. B., Somners G. F.:Laboratory Experiments in Biological Chemistry. Academic Press, New York 1944.

    Google Scholar 

  • Usami S., Fumio C.: Submerged culture production and utilization of cellulase.Asahi Kogyo Kenkya Mokoku 13, 237 (1967).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gupta, J.K., Gupta, Y.P. Properties of cellulase fromTrichoderma viride . Folia Microbiol 24, 269–272 (1979). https://doi.org/10.1007/BF02926459

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02926459

Keywords

Navigation