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Genetic mapping of the regionc of the bacteriophage G101 (Pseudomonas aeruginosa)

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Abstract

Morphological mutants of the c type of the bacteriophage G101 (Pseudomonas aeruginosa) were isolated after mutagenesis with hydroxylamine. Complementation analysis of 27c mutants showed that thec region is formed by at least two genes. Two types ofc mutants were obtained. One of them (cI26) behaves analogously to a mutant in the gene controlling the synthesis of the repressor of phage λ. The second type of thec mutants (cII1, cII18) specifies a gene having probably an auxiliary function in the “c” region. According to the low frequency of recombination between the genes cI26 and cII18 (1.37 recombination units), these genes responsible for lysogenization are localized in a short region of the chromosome.

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References

  • Bertani L. E.: Characterization of clear mutants belonging to Z bacteriophage P2.Virology 71, 85 (1976)

    Article  PubMed  CAS  Google Scholar 

  • Chakrabarty A. M., Niblach J. F., Gunsalus I. S.: A-phage initiated polysaccharide depolymerase inPs putida.Virology 32, 523 (1967).

    Article  Google Scholar 

  • Egan J. B., Holloway B. W.: Genetic studies on lysogeny inPa. aeruginosa.Austral. J. Exp. Biol. Med. Sci. 39, 9 (1961).

    Article  CAS  Google Scholar 

  • Gough H.: Establishment mode repressor synthesis blunts phage P22 antirepressor activity.J. Mol. Biol. 111, 56 (1977).

    Article  Google Scholar 

  • Holloway B. W.: Genetics of Pseudomonas.Bacteriol. Rev. 33, 419 (1969).

    PubMed  CAS  Google Scholar 

  • Holloway B. W., Krishnapillai V.: Bacteriophages and bacteriocins, p. 99 inGenetic and Biochemistry of Pseudomonas (P. H. Clarke, M. H. Richmond, Eds.). Willey, London 1975.

    Google Scholar 

  • Holloway B. W., Van De Putte P.:Replication and Recombination of Genetic Material (J. W. Peacock, R. D. Brock, Eds.), p. 175. Australian Academy of Science, Canberra 1968.

    Google Scholar 

  • Holloway B. W., Egan J. B., Monk M.: Lysogeny inPs. aeruginosa.Austral. J. Exp. Biol. Med. Sci. 38, 321 (1960).

    Article  CAS  Google Scholar 

  • Holloway B. W., Krishnapillai V., Stanisich V. A.: Pseudomonas genetics.Ann. Rev. Genet. 5, 425 (1971).

    Article  PubMed  CAS  Google Scholar 

  • Kaiser A. D.: Mutations in a temperate bacteriophage affecting hisability to lysogenizeE. coli.Virology 3, 42 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Krishnapillai V.: The use of bacteriophages for differentiating plasmids ofPs. aeruginosa.Genet. Res. 23, 327 (1974).

    PubMed  CAS  Google Scholar 

  • Levine M.: Mutations in the temperate phage P22 and lysogeny in Salmonella.Virology 3, 22 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Levine M., Smith H. O.: Sequential gene action in the establishment of lysogeny.Science 146, 1581 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Miller R. V., Pemberton J. M., Richards K. E.: F116, D3 and G101: Temperate bacteriophages ofPseudomonas aeruginosa.Virology 59, 566 (1974).

    Article  PubMed  CAS  Google Scholar 

  • Ptashne M.: Repressor and its action, p. 221 inThe Bacteriophage lambda (A. D. Heshey, Ed.). Cold Spring Harbor Laboratory, Cold Spring Harbor 1971.

    Google Scholar 

  • Tessman I., Poddar R. K., Kumar S.: Identification of the altered bases in mutated single-stranded DNA. I.In vitro mutagenesis by hydroxylamine, ethyl methanesulphonate and nitrous acid.J. Mol. Biol. 9, 352 (1964).

    Article  PubMed  CAS  Google Scholar 

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The author wishes to thank Dr. J. Soška for his help, advice and suggestions concerning both the experimental work and preparation of the manuscript.

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Španová, A. Genetic mapping of the regionc of the bacteriophage G101 (Pseudomonas aeruginosa). Folia Microbiol 24, 217–223 (1979). https://doi.org/10.1007/BF02926451

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