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Utilization of cystine by dermatophytes on glucose-peptone media

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Abstract

All the 16 strains of dermatophytes tested here metabolized cystine (3 mmol/L) in two glucose-peptone media with a different C: N ratio. Cystine was utilized as a sulfur source and, in addition, as a carbon and nitrogen source, in parallel with growth. Excess sulfur was excreted to the medium after its oxidation as inorganic sulfate and sulfite. In a physiologically alkaline medium the growth was fast and was accompanied by a pH increase and cystine was utilized intensively. Eleven species used up all cystine available. Sulfate was the main oxidation product, sulfite was produced at a low concentration, at the beginning of growth in particular. Only traces of thiol compounds (cysteine) were present in the medium. In a physiologically acid medium growth was soon limited by a decreased pH (below 5.0) but cystine continued to be utilized at an identical rate. All cystine was used up by 5 species. The tendency to produce sulfite in addition to sulfate further increased and sulfite was often the predominant product. Concentrations of thiol compounds were also substantially higher. Thus, dermatophytes can utilize cystine even under conditions that do not support good growth and increase the sulfite production.

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References

  • Cecil R., McPhee J.R.: A kinetic study of the reactions of some disulphides with sodium sulphite.Biochem. J. 60, 496–506 (1955).

    PubMed  CAS  Google Scholar 

  • Chattaway F.W., Toothill C., Barlow A.J.E.: The amino acid metabolism ofMicrosporum canis.J. Gen. Microbiol. 28, 721–732 (1962).

    PubMed  CAS  Google Scholar 

  • Danew P., Marquardt I., Friedrich E., Aurich H.: Aminosäureassimilataon und -sekretion bei Dermatophyten. 2. Aminosäureabbau und Myzelwachstum beiMicrosporum gypseum nach Wachstum in Nährlösung mit verschiedenen Aminosäuren.Mykosen 24, 561–565 (1981).

    PubMed  CAS  Google Scholar 

  • Danew P., Friedrich E., Farr W., Aurich H., Haschen R.J.: Abbau und Assimilation von Glukose und Aminosäuren durchMicrosporum gypseum.Mykosen 25, 143–150 (1982).

    PubMed  CAS  Google Scholar 

  • Kunert J.: Formation of sulphate, sulphite and S-sulphocysteine by the fungusMicrosporum gypseum during growth on cystine.Folia Microbiol. 20, 142–151 (1975).

    Article  CAS  Google Scholar 

  • Kunert J.: Utilization ofl- andDl-cystine by the fungusMicrosporum gypseum.Folia Microbiol. 27, 390–394 (1982).

    Article  CAS  Google Scholar 

  • Kunert J.: Cystine metabolism in the dermatophyteMicrosporum gypseum. I. Utilization of cystine in media containing glucose.Acta Univ. Olomuc. Olomouc Fac. Med. 108, 39–52 (1985a).

    CAS  Google Scholar 

  • Kunert J.: Cystine metabolism in the dermatophyteMicrosporum gypseum. II. Utilization of cystine in media containing peptides and proteins.Acta Univ. Olomuc. Olomouc Fac. Med. 111, 37–47 (1985b).

    CAS  Google Scholar 

  • Kunert J.: Metabolism of sulfur-containing amino acids in the dermatophyteMicrosporum gypseum. I. Neutral amino acids.J. Basic Microbiol. 25, 31–37 (1985c).

    Google Scholar 

  • Kunert J.: Utilization of cystine by dermatophytes in gelatin medium.Folia Microbiol. 33, 121–128 (1988).

    Article  CAS  Google Scholar 

  • Milligan B., Swan J.M.: Bunte salts (RSSO3Na).Rev. Pure Appl. Chem. 12, 72–94 (1962).

    CAS  Google Scholar 

  • Schaper H.G., Ziegler H.: Untersuchungen über den Cystinstoffwechsel der Dermatophyten.Mykosen 14, 589 (1971).

    Google Scholar 

  • Schaper H.G., Ziegler H.: Untersuchungen zum Schwefelstoffwechsel der Dermatophyten.Proc. 3rd Mycol. Symp., Szczecin, p. 21 (1972).

  • Stricks W., Kolthoff J.M.: Equilibrium constants of the roaction of sulfite with cystine and with dithiodiglycollic acid.J. Amer. Chem. Soc. 73, 4569–4574 (1951).

    Article  CAS  Google Scholar 

  • Ziegler H., Reichmask G.: Über den Schwefelstoffwechsel vonMicrosporum canis.Mykosen 11, 903–907 (1968).

    PubMed  CAS  Google Scholar 

  • Ziegler H., Böhme H., Reichmann G.: Stoffwechselphysiologische Untersuchungen über den Abbau von Proteinen durchMicrosporum gypseum und M. canis.Dermat. Mschr. 155, 835–856 (1969).

    CAS  Google Scholar 

  • Ziegler H., Böhme H.: Biologie und Chemie der Keratinisation sowie des Keratinabbaues durch vorzugsweise pathogene Pilze.Biol. Rundschau 15, 27–40 (1977).

    CAS  Google Scholar 

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  1. Department of Biology, Medical Faculty, Palacký University, 775 15, Olomouc, Czechoslovakia

    J. Kunert

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Kunert, J. Utilization of cystine by dermatophytes on glucose-peptone media. Folia Microbiol 33, 188–197 (1988). https://doi.org/10.1007/BF02925904

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