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Optimization of growth ofEscherichia alcalescens with respect to its aspartate ammonia-lyase activity

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Abstract

Aspartate ammonia-lyase activity ofEscherichia alcalescens was positively affected by the composition of the culture medium and by a higher intensity of aeration. By using the supernatant obtained from autolyzed baker’s yeast, the aspartate ammonia-lyase activity increased two-fold (about 90 μkat/g wet biomass) as compared to commercial yeast autolyzates.

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Authors and Affiliations

  1. Research Institute for Biofactors and Veterinary Drugs, Kouřim Research Pilot Plant, 281 61, Kouřim, Czechoslovakia

    I. Pšenička, V. Malaník, I. Sojková & M. Malaníkova

  2. Department of Biochemistry and Microbiology, Institute of Chemical Technology, 166 28, Prague 6, Czechoslovakia

    M. Marek

Authors
  1. I. Pšenička
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  2. V. Malaník
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  3. I. Sojková
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  4. M. Malaníkova
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  5. M. Marek
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Additional information

The authors thank Mr. J. Tomíček (Food Industry Research Institute, Prague) for a sample of the yeast autolyzate of the Kolín type.

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Pšenička, I., Malaník, V., Sojková, I. et al. Optimization of growth ofEscherichia alcalescens with respect to its aspartate ammonia-lyase activity. Folia Microbiol 33, 178–182 (1988). https://doi.org/10.1007/BF02925902

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  • DOI: https://doi.org/10.1007/BF02925902

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