Abstract
The validity of the principle of homeoviscous adaptation forBacillus subtilis was tested by comparing fluorescence aniaotropy (1,6-diphenyl-1,3,5-hexatriene) and electron-spin resonance (16-doxylstearate) measurements carried out in isolated plasma membranes and in phospholipid fractions. The physical measurements were supplemented by fatty-acid analysis. The results support our previous findings on intact cells. The thermoadaptive mechanism ofB. subtilis manifested as an increase in relative proportion of branchedanteiso-C15 andanteiso-C17 fatty acids, are not strong enough to compensate for the marked physical change of membrane fluidity induced by temperature decrease.
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References
Bishop D.G., Rutberg L., Samuelson B.: The chemical composition of the cytoplasmic membrane ofBacillus subtilis.Eur. J. Biochem. 2, 448–453 (1967).
Bohin J. P., Bohin A., Schaeffer P.: Increased nitrate reductase activity as a sign of membrane alternation in early blocked asporogenous mutants ofBacillus subtilis.Biochemie 58, 99–108 (1976).
Cossins A.R., Sinenski M.: Adaptation of membranes to temperature, pressure and exogenous lipids, p. 1–20 inPhysiology of Membrane Fluidity, Vol. II (M.Shnitzki, ed.). CRC Press, Boca Raton (Florida) 1984.
Glass R.L.: Alcoholysis saponification and the preparation of fatty acids methylesters.Lipids 6, 919–926 (1971).
Kaback H.R.: Bacterial membranes, p. 99–107 inMethods in Enzymology, Vol. 22. Academic Press, New York 1971.
Kaneda T.: Positional distribution of fatty acids in phospholipids fromBacillus subtilis.Biochim. Biophys. Acta 270, 32–39 (1972).
Lindgren V., Holmgren E., Rutberg L.:Bacillus subtilis mutant with temperature-sensitive net synthesis of phosphatidylethanolamine.J. Bacteriol. 132, 473–484 (1977).
Quint J.F., Fulco A.J.: The biosynthesis of unsaturated fatty acids by bacilli.J. Biol. Chem. 248, 6885–6895 (1973).
Radin N.S.: Extraction of tissue lipids with a solvent of low toxicity, p. 5–7 inMethods in Enzymology, Vol. 72. Academic Press, New York 1981.
Rottem S., Markowitz O., Razin S.: Thermal regulation of the fatty acid composition of lipopolysaccharides and phospholipidsof Proteus mirabilis.Eur. J. Biochem. 85, 451–456 (1978).
Seelig J.:Spin Labelling, Theory and Applications (L.J. Berliner, ed.), p. 373. Academic Press, New York 1976.
Silvius J.R., Mak N., McElhaney R.N.: Why do prokarytes regulate membrane fluidity?, p. 213 inMembrane Fluidity: Biophysical Techniques and Cellular Regulations (M.Kates, A.A.Kuksis, eds.). Humana Press Clifton, New York 1980.
Sinensky M.: Homeoviscous adaptation — a homeostatic process that regulates the viscosity of membrane lipids inEscherichia coli.Proc. Nat. Acad. Sci. USA 71, 522–525 (1974).
Svobodová J., Svoboda P.: Cytoplasmic membrane fluidity measurements on intact living cells ofBacillus subtilis by fluorescence anisotropy of 1,6-diphenyl-1,3,5-hoxatriene.Folia Microbiol. 33, 1–9 (1988a).
Svobodová J., Svoboda P.: Membrane fluidity inBacillus subtilis. Physical change and biological adaptation.Folia Microbiol. 33, 161–169 (1986).
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Svobodová, J., Julák, J., Pilař, J. et al. Membrane fluidity inBacillus subtilis. Validity of homeoviscous adaptation. Folia Microbiol 33, 170–177 (1988). https://doi.org/10.1007/BF02925901
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DOI: https://doi.org/10.1007/BF02925901