Abstract
Eight strains ofEscherichia coli, isolated from patients with a urinary tract infection were investigated for production of hemolysin. Six of these produced hemolysin and one revealed maximum hemolytic activity. Three urinary and two faecal isolated were positive for mannoseresistant hemagglutination. One isolate positive for hemagglutination and giving maximum hemolytic activity was then used. Hemolysin was present in the supernatant broth and the medium of choice to obtaine the optimum yield was the alkaline meat extract broth followed by brain heart infusion broth. The highest yield appeared in the exponential phase of growth. Hemolysin is a heat-labile protein, being produced optimally at pH 8. A three-stage procedure was the best method for its purification.
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The authors are thankful to the Chairman,Department of Microbiology, Punjab University (Chandigarh), for providing the necessary facilities for this study.
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Asnani, P.J., Bhatnagar, N. & Bhandari, S. Production and purification ofEscherichia coli hemolysin. Folia Microbiol 33, 393–400 (1988). https://doi.org/10.1007/BF02925850
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DOI: https://doi.org/10.1007/BF02925850