Abstract
Sterol composition was examined during batch growth on complex media containing ethanol, molasses or glucose as the carbon source. The molasses-grown cells exhibited a balanced sterol composition throughout growth, maintaining the proportion of eigosterol to 24:28-dehydroergosterol equal to 1.4. The negative effect of glucose on sterol synthesis manifested itself by decreasing the accumulation of 24:28-dehydroergosterol and total sterols but not of ergosterol. Using ethanol as the sole carbon source, a large amount of 24:28-dehydroergosterol accumulated, partly at the expense of other sterols. The gradual addition of nitrogon source during growth significantly decreased the accumulation of ergosterol, 24:28-dehydroergosterol and of total sterols. A general scheme of regulation of sterol synthesis in baker’s yeast is presented.
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The excellent technical assistance of J. Honzíková and M. Vyletalová is gratefully acknowledged.
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Novotný, Č., Běhalová, B., Strtjžinský, R. et al. Sterol composition of a δ5,7-sterol-rich strain ofSaccharomyces cerevisiae during batch growth. Folia Microbiol 33, 377–385 (1988). https://doi.org/10.1007/BF02925848
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DOI: https://doi.org/10.1007/BF02925848