Abstract
Indirect fluorescent antibody method was used to demonstrate the localization ofA. clavatus ribonuclease in apical cell ends. Using protoplasts, ribonuclease was found to be present mainly in the periplasmic space.
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Bezborodova S.I., Borodaeva L.I., Patikova L.I.: Capacity of lower fungi to synthesize extracellular RNAase. (In Russian)Microbiology 37, 10–14 (1968).
Bezborodova S.I., Beletskaya O.P., Grishchbnko V.M.: Large-scale purification, crystallization and physico-chemical properties of extracellular guanyl-RNA-ases C2 and Pch1. (In Russian)Biochemistry 42, 1556–1566 (1977).
Kulikoya A.K., Letunova E.V., Martynovich L.I.: Protoplast formation and localization of enzymes in the mycelium ofAspergillus clavatus. (In Russian)Appl. Biochem. Microbiol. 21, 587–591 (1985).
Osterman L.A.:Investigation of Biological Macromolecules by Electrofocussing, Immunoelectrophoresis and Radioisotopic Techniques. (In Russian) Nauka, Moscow 1983.
Tonomura K., Tanabe O.: Localization of cell-bound α-amylase inAspergillus oryzae demonstrated by fluorescent antibody technique.J. Bacterial. 87, 226–227 (1964).
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Letunova, E.V., Ermekbaeva, L.A., Kozlovskaya, L.N. et al. Determination of localization of ribonclease inAspergillus clavatus . Folia Microbiol 33, 360–362 (1988). https://doi.org/10.1007/BF02925845
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DOI: https://doi.org/10.1007/BF02925845