Abstract
Aspergillus ochraceus produced xylanase inductively in washed glucose-grown mycelia incubated with methyl β-d-xyloside. The production of xylan endo-l,4-β-xylosidase (xylanase) was 4.2 times greater than that obtained in xylan medium. The inducer was not metabolized and was most active at a concentration of 0.3 g/L with an incubation period of 16 h. The uptake of the inducer by the mycelia seemed to be energy-dependent. Methyl α-d-xyloside repressed xylanase synthesis. Glucose, cyeloheximide, actinomycin D and eAMP were found to inhibit xylanase induction by methyl β-D-xyloside.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Biely P., Krátký Z., Vrsanska M., Urmanicova D.: Induction and inducers of endo-l,4-β-xylanase in the yeastCryptococcus albidus.Eur. J. Biochem. 108, 323–329 (1980).
Biely P., Krátký Z.: Inducible β-xyloside permease as a constituent of the xylan-degrading enzyme system of the yeastCryptococcus albidus.Eur. J. Biochem. 112, 367–373 (1980).
Biely P.: Catabolite repression of β-xylanase synthesis in the yeastCryptococcus albidus.Biológia (Bratislava) 37, 799–807 (1982).
Biswas S.R., Mishra A.K., Nanda G.: Xylanolytic activity ofAspergillus ochraceus-42.Curr. Sci. 55 (7) 351–352 (1986).
Biswas S.R., Mishra A.K., Nanda G.: Studies on xylanase production by a strain ofAspergillus ochraceus under shake culture conditions.J. Microbiol. Biotechnol. in press (1988).
Cooper R.M., Wood R.K.S.: Induction of synthesis of extracellular cell wall degrading enzymes in vascular wilt fungi.Nature 246, 309–311 (1973).
Herbert D., Phipps P.J., Strange R.E.: Chemical analysis of microbial cells, p. 289 inMethods in Microbiology, Vol. 5B (1971).
Kusakabe I., Yasui T., Kobayshi T.: Xylanase induction inStreptomyces sp.Nippon Nogeikagaku 49, 383–385 (1975).
Lilley G., Rowley B.I., Bull A.T.: Synthesis of l,3-β-glucanase in aStreptomyces sp.J. Appl. Chem. Biotech. 24, 677–686 (1974).
Nisizawa T., Suzuki H., Nisizawa K.: Catabolite repression of cellulase formation inTrichoderma viride.J. Biochem. 71, 999–1007 (1972).
Panbangred W., Shinmyo A., Kinoshita S., Okada H.: Purification and properties of endoxylanase produced byBacillus pumilus.Agric. Biol. Chem. 47, 957–963 (1983).
Perlman R.L., de Crombrugghe B., Pastan I.: Cyclic AMP regulates catabolic and transient repression inE.coli.Nature 223, 810–812 (1969).
Reid G.C., Robb F.T., Woods D.R.: Regulation of extracellular collagenase production inAchromobacter iophagus.J. Gen. Microbiol. 109, 149–154 (1978).
Somogyi M.: Notes on sugar determination.J. Biol. Chem. 195, 19–23 (1952).
Santos T., Villanueva J.R., Nombella C.: Production and catabolic repression ofPenicillium italicum.J. Bacteriol. 129, 52–58 (1977).
Yasui T., Nakanishi K., Kobayashi T.: Induction of xylanase by β-d-xylosides and inhibition of xylanase induction by α-d-xylosides inStreptomyces sp.Hakkokogaku 58, 79–84 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Biswas, S.R., Mishra, A.K. & Nanda, G. Induction of xylanase inAspergillus ochraceus . Folia Microbiol 33, 355–359 (1988). https://doi.org/10.1007/BF02925844
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02925844