Abstract
A method is described for spectrophotometric monitoring the degradation of the herbicide bromoxynil by cell-free extracts ofStreptomyces felleus. The method involves a decrease in absorbance at 286 nm (absorption maximum of bromoxynil) that can be ascribed most probably to the cleavage of the aromatic ring of the bromoxynil molecule. Conditions necessary for measuring this degradation together with physico-chemical features of the degradation indicate that the reaction(s) is seemingly catalyzed by an Fe2+-dependent dioxygenase whose activity was not, however, detected in cell-free extracts of a bromoxynil-sensitive mutantof S. felleus as well as other bromoxynil-sensitive streptomycete strains.
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We would like to express our thanks to Dr. J. Teisinger from theInstitiute of Hygiene and Epidemiology, Prague, for his interest and fruitful discussions.
Part 3 of the series Resistance against the herbicide bromoxynil inStreptomyces felleus. Part 2:Folia Microbiol. 32, 305-313 (1987).
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Neužil, J., Krištůfek, V. & Blumauebová, M. Enzymic degradation of bromoxynil by cell-free extracts ofStreptomyces felleus . Folia Microbiol 33, 349–354 (1988). https://doi.org/10.1007/BF02925843
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DOI: https://doi.org/10.1007/BF02925843