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Comparative study of nitrogen and oxygen metabolism enzymes in Yugoslav cultivars of alfalfa (Medicago sativa L.)

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Biologia Plantarum

Abstract

Four Yugoslav cultivars of alfalfa were investigated in order to determine nitrogen fixing (nitrogenase), nitrogen assimilation (nitrate reductase, glutamine synthetase, glutamate dehydrogenase) and antioxidant (Superoxide dismutase, catalase, peroxidase) enzymes activities. The level of lipid peroxidation and protein content were also investigated. On the basis of the results obtained a resistant cultivar with high nitrogen fixing and a cultivar with high nitrogen assimilation abilities were chosen. The cultivar with high nitrogen fixing ability had high activities of nitrogenase, Superoxide dismutase, peroxidase and catalase, and also a low level of lipid peroxidation. The cultivar with high assimilation ability had high activities of nitrate reductase, glutamine synthetase and glutamate dehydrogenase and high soluble protein content.

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Abbreviations

NR:

nitrate reductase

GS:

glutamine synthetase

GDH:

glutamate dehydrogenase

NG:

nitrogenase

AA:

amino acids

SOD:

Superoxide dismutase

P:

peroxidase

C:

catalase

LP:

lipid peroxidation

MDA:

malonyldialdehyde

TBA:

thiobarbituric acid

LSD:

least significant difference

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Authors and Affiliations

  1. Institute of Chemistry, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradoviča 3, 21000, Novi Sad, Yugoslavia

    D. Štajner, M. Popovič & O. Gašič

  2. Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradoviča 3, 21000, Novi Sad, Yugoslavia

    B. Mišković

Authors
  1. D. Štajner
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  2. M. Popovič
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  3. O. Gašič
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  4. B. Mišković
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Štajner, D., Popovič, M., Gašič, O. et al. Comparative study of nitrogen and oxygen metabolism enzymes in Yugoslav cultivars of alfalfa (Medicago sativa L.). Biol Plant 34, 77–83 (1992). https://doi.org/10.1007/BF02925794

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  • DOI: https://doi.org/10.1007/BF02925794

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