Abstract
The primary transformant of spring rapeseed cv. HM-81 contained TL- and TR-DNA of agropine plasmid pRi ofAgrobacterium rhizogenes 15834. The presence of TL-DNA corresponds to visible transformed phenotype in its progeny; the leaves are wrinkled and the plants are shorter than normal plants. R1 R2 and R3 generations have mostly transformed phenotype. The normal phenotype appears in a low frequency in F1 generation. Autogamised F1 plants segregate in F2 transformed and normal phenotype in 3:1 ratio. It is possible to suppose that TL-DNA is present in two differentloci of one pair of homologic chromosomes. The recombination frequency is 12 % (microsporogenesis) or 6 % (microsporogenesis and macrosporogenesis). In some crosses the transformed phenotype has a maternal type of inheritance. Maternal inheritance influences also several growth characteristics,e.g. length of plants and number of seeds/pods.
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Dusbábková, J., Nečásek, J., Hrouda, M. et al. Segregation in the progeny of transformed rapeseed (Brassica napus). Biol Plant 34, 53–61 (1992). https://doi.org/10.1007/BF02925790
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DOI: https://doi.org/10.1007/BF02925790