Skip to main content
SpringerLink
Log in

Effects of mineral composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocksin vitro

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The influence of the mineral composition and acidity of media, saccharose level, brand and quantity of agar onin vitro shoot rooting of P 60 and P 2 apple and quince S 1 root-stocks were compared. Among the tested salt compositions (full WMP and 1/2 WMP, full MS and 1/2 MS) the most suitable for rooting was composition of WMP. Further modifications in quantity of nitrogen, H3BO3 CaCl2 and MgSO4 of WMP medium did not have a positive effect on rooting. 30 g l−1 saccharose gave better results than 20 g l−1. Acidity of WMP medium tested in a pH range of 4.0 to 6.5 did not affect rooting of P 60 rootstock but media adjusted to pH 4.5 and 5.0 were better for rooting of recalcitrant P 2 rootstock. Among 4 agar brands (Difco Bacto, Oxoid No.1, Japanese commercial fibre and Japanese commercial powder) tested at 6 g l−1, the most effective was the last one, but its quantity had no effect in the range of 3 to 10 g l−1 for rooting of P 60 rootstock.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Blazich, F.A., Wright, R.D., Schaffer, H.E.: Mineral nutrient status of “Convexa” holly cuttings during intermittent mist propagation as influenced by exogenous auxin application. — J. amer. Soc. hort. Sci.108: 425–429, 1983.

    CAS  Google Scholar 

  • Debergh, P.C.: Effects of agar brand and concentration on the tissue culture medium. — Physiol. Plant.59: 270–276, 1983.

    Article  CAS  Google Scholar 

  • Dunstan, D.: Transplantation and post-transplantaion of micropropagated tree-fruit rootstocks. — Proc. int. Plant. Propag. Soc.31: 39–45, 1981.

    Google Scholar 

  • Eliasson, L.: Effect of nutrients and light on growth and root formation inPisum sativum cuttings. — Physiol. Plant.43: 13–18, 1978.

    Article  CAS  Google Scholar 

  • Hammerschlag, F.: Factors influencingin vitro multiplication and rooting of the plum rootstock Myrobalan(Prunus cerasifera Ehrh.). — J. amer. Soc. hort. Sci.107: 44–47, 1982.

    Google Scholar 

  • Geneve, R.L., Heuser, C.W.: The effect of IAA, IBA, NAA and 2.4-D on root promotion and ethylene evolution inVigna radiata cuttings. — J. amer. Soc. hort Sci.107: 202–205, 1982.

    CAS  Google Scholar 

  • Hasegawa, P.M.: Factors affecting snoot and root initiation from cultured rose shoot tips. — J. amer. Soc. hort. Sci.105: 216–220, 1980.

    CAS  Google Scholar 

  • Jarvis, B.C., Ali, A.H.N., Shaheed, A.I.: Auxin and boron in relation to the rooting response and ageing of mung bean cuttings. — New Phytol.95: 505–518, 1983.

    Article  Google Scholar 

  • Lewis, D.H.: Boron, lignification and the origin of vascular plants — a unified hypothesis. — New Phytol.84: 209–229, 1980.

    Article  CAS  Google Scholar 

  • Lloyd, G., McCown, B.: Commercially-feasible micropropagation of mountain laurel,Kalmia latifolia, by use of shoot-tip culture. — Proc. Int. Plant Propag. Soc.30: 421–427, 1980.

    Google Scholar 

  • Machnik, B., Orlikowska, T.:In vitro propagation of P 22 malus clonal rootstock. — Fruit Sci. Rep.8: 173–177, 1981.

    Google Scholar 

  • McCown, B.H., Lloyd, G.: Woody plant medium (WPM) — a mineral nutrient formulation for microculture of woody plant species. — HortScience16: 453, 1981.

    Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant.15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Orlikowska, T.: Propagation of quince S 1(Cydonia oblonga Mill.)in vitro. — Fruit Sci. Rep.15: 157–165, 1988.

    Google Scholar 

  • Orlikowska, T.: Propagationin vitro of P 60 — new Polish clonal apple rootstock. — Fruit Sci. Rep.18: 1–5, 1991.

    Google Scholar 

  • Pua, E.-C, Chong, C.: Regulation ofin vitro shoot and root regeneration in “Macspur” apple by sorbitol (D-glucitol) and related carbon sources. — J. amer. Soc. hort. Sci.110: 705–709, 1985.

    CAS  Google Scholar 

  • Singha, S.: Influence of agar concentration onin vitro shoot proliferation ofMalus sp.“Almey” andPyrus communis “Seckel”. — J. amer. Soc. hort. Sci.107: 657–660, 1982.

    Google Scholar 

  • Welander, M.:In vitro rooting of the apple rootstock M 26 in adult and juvenile growth phases and acclimatization of the plantlets. — Physiol. Plant.58: 231–238, 1983.

    Article  CAS  Google Scholar 

  • Zatyko, J.M., Molnar, I.: Advenitious root formation of chokeberry(Aronia melanocarpa Elliot) influenced by the pH of medium. — Fruit Sci. Rep.17: 21–27, 1990.

    Google Scholar 

  • Zimmerman, R.H.: Factors affectingin vitro propagation of apple cultivars. — Acta Hort131: 171–178, 1983.

    Google Scholar 

  • Zimmerman, R.H., Fordham, I.: Simplified method for rooting apple cultivarsin vitro. — J. amer. Soc. hort. Sci.110: 34–38, 1985.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute of Pomology and Floriculture, 96-100, Skierniewice, Poland

    T. Orlikowska

Authors
  1. T. Orlikowska
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Orlikowska, T. Effects of mineral composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocksin vitro . Biol Plant 34, 45–52 (1992). https://doi.org/10.1007/BF02925789

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02925789

Keywords

Search

Navigation