Abstract
The effect of twelve amino acids and lactalbumin hydrolysate in concentration of 200 mg 1−1 on rooting of the dwarf apple rootstocks P 2 and P 60 was testedin vitro. Arginine, omithine, glutamic acid and glycine enhanced root number of the P 60 rootstock; proline and lactalbumin hydrolysate were neutral; and asparagine, tyrosine, methionine, cysteine and glutamine lowered the root number. Tyrosine, methionine, cysteine and glutamine reduced almost completely rooting of P 60. In the recalcitrant P 2 rootstock aspartic acid, glutamic acid and omithine significantly enhanced the number of roots and rooted shoots, arginine and tryptophan increased the root number only slightly, asparagine was neutral, and proline reduced the root number.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Behrend, J., Mateles, R.I.: Nitrogen metabolism in plant cell suspension cultures. I. Effect of amino acids on growth. — Plant Physiol.56: 584–589, 1975.
Boxus, Ph.: Development ofin vitro propagation methods applied to fruit trees. — In: Conf. Fruit Tree Biotechnology. Pp. 8–9. Paris 1986.
Czynczyk, A., Olszewska, B.: Growth and yielding of 3 apple cultivais on rootstocks of Polish and foreign breeds. — Fruit Sci. Rep.17: 65–75, 1990.
Druart, P.: Contribution a l'Elaboration de Techniques de Production en Massein Vitro d'Especes Ligneuses Utilisables en Culture Fruitiere. — Dissertation. Faculté des Sciences Agronomiques de l'Etat Gembloux 1986.
Druart, P., Kevers, C., Boxus, Ph., Gaspar, T.:In vitro promotion of root formation by apple shoots through darkness effect on endogenous phenols and peroxidases. — Z. Pflanzenphysiol.108: 429–436, 1983.
Duron, M., Decourtye, L., Druart, P.: Quince(Cydonia oblonga Mill.). — In: Bajaj, Y.P.S. (ed.): Biotechnology in Agriculture and Forestry. Vol. 5. Trees II. Pp. 42–58. Springer-Verlag, Berlin — Heidelberg — New York — Tokyo 1989.
Faye, M., Ourry, A., Saidali-Savi, C., Dargent, R., Boucaud, J., David, A.: Effects of glutamine and K-glutamate on assimilation of [15N]-nitrate during auxin treatment for root formationin vitro (Pinus pinaster). — Physiol. Plant.76: 277 -282, 1989.
Haissig, B.E.: Metabolic process in adventitious rooting of cuttings. — In: Jackson, B.M. (ed.): New Root Formation in Plants and Cuttings. Pp. 141–189. Martinus Nijhoff, Dordrecht 1986.
Jones, O.P.:In vitro propagation of tree crops. — In: Mantell, S.H., Smith, H. (ed.): Plant Biotechnology. Pp. 139–159. Cambridge University Press, Cambridge 1983.
Kamada, H., Harada, H.: Influence of several growth regulators and amino acids onin vitro organogenesis ofTorenia fournieri Lind. — J. exp. Bot.30: 27–36, 1979.
Kirby, E.G., Leustek, T., Lee, M.S.: Nitrogen nutrition. — In: Bonga, J.M. Durzan, D.J. (ed.).: Cell and Tissue Culture in Forestry. Vol. 1. Pp. 67–88. Martinus Nijhoff, Dordrecht 1987.
Lloyd, G., McCown, B.: Commercially-feasible micropropagation of mountain lauvel,Kalmia latifolia, by use of shoot-tip culture. — Proc. Intern. Plant Propag. Soc.30: 421–427, 1981.
Marion —, Poll, A., Caboche, M.: Relationship between auxin and amino acid metabolism of tobacco protoplast-derived cells. — Plant Physiol.75: 1048–1053, 1984.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant.15: 473–497, 1962.
Orlikowska, T.: Propagation of quince S 1(Cydonia oblonga Mill.)in vitro. — Fruit Sci. Rep.15: 157–165, 1988.
Orlikowska, T.: Propagationin vitro of P 60 — new Polish clonal apple rootstock. — Fruit Sci. Rep.18: 1–5, 1991.
Orlikowska, T.: Effects of mineral composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocksin vitro. — Biol. Plant.34: 45–52, 1992.
Raghavan, V.: Nutrition, growth and morphogenesis of plant embryos. — Biol. Rev.41: 1–58, 1966.
Suzuki, T., Kohno, K.: Changes in nitrogen levels and freee amino acids in rooting cuttings of mulberry(Morus alba). — Physiol. Plant.59: 455–460, 1983.
Welander, T.: Influence of nitrogen and sucrose in the medium and of irradiance of the stock plants on root formation inPelargonium petioles grownin vitro. — Physiol. Plant.43: 136–141, 1978.
Zagaja, S.W., Jakubowski, T., Pieklo, A., Przybyla, A.: Preliminary evaluation of new clones of apple rootstocks. — Fruit Sci. Rep.16: 205–213, 1989.
Zimmerman, R.H.: Temperate Fruits: Apple. — In: Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (ed.). Handbook of Plant Cell Culture. Vol. 2. Pp. 369–395. Macmillan-New York. 1983.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Orlikowska, T. Effect of amino acids on rooting of apple dwarf rootstocksin vitro . Biol Plant 34, 39–44 (1992). https://doi.org/10.1007/BF02925788
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02925788