Abstract
When distantly related plants are crossed, embryos abort on the mother plant. One of the methods used to rescue the embryo is to cultivate the ovulein vitro. When the ovule is precociously detached from the mother plant, survival rate of the embryo is very low. To increase the chance of survival as well as the growth of the embryo, the medium was supplemented by different extracts of plants after either autoclaving or filtering. Autoclaved tomato extract seemed to have no simulating effect in spite of reports from different authors. Filtered coconut milk increased growth and filtered cucumber extract improved survival. Other new extracts had a stimulating action on both growth and survival and are of interest for further study.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lagriffol, J., Monnier, M.: Etude de divers parametres en vue de la culturein vitro des ovules deCapsella bursa-pastoris. — Can. J. Bot.61: 3471–3477, 1983.
Lagriffol, J., Monnier, M.: Effects of endosperm and placenta on development ofCapsella embryos in ovules cultivatedin vitro. — J. Plant Physiol.118: 127–137, 1985.
Matsubara, S., Koshimizu, K.: Factors with cytokinin activity in youngLupinus seeds and their partial purification. — Bot Mag. (Tokyo)79: 389–396, 1966.
Monnier, M.: Culturein vitro de l'embryon immature deCapsella bursa-pastoris Moench. — Rev. Cyt. Biol. vég.39: 1–120, 1976.
Monnier, M.: Culture of mature ecotyledonous embryos ofPhaseolus vulgaris and the nutritional role of cotyledons. — Amer. J. Bot.69: 896–903, 1982.
Monnier, M.: Survival of young immatureCapsella embryos culturedin vitro. — J. Plant Physiol.115: 105–113, 1984.
Monnier, M., Lagriffol, J.: Effect of ovular tissue on the development ofCapsella embryos cultivatedin vitro. — J. Plant Physiol.122: 17–24, 1986.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue culture. — Physiol. Plant5: 473–497, 1962.
Nakajima, T., Doyoma, Y., Matsumoto, H.:In vitro culture of excised ovules of white cloverTrifolium repens L. — Jap. J. Breed19: 373–378, 1969.
Nitsch, J.: Action du jus de tomate sur la croissance de certains tissus et organes végétaux. — Bull. Soc. bot. Fr.101: 433–440, 1954.
Norstog, K.: The growth and differentiation of cultured barley embryos. — Amer. J. Bot.48: 876- 884, 1961.
Schaffner, M.: The embryology of the shepherd's purse. — Ohio Natur.7: 1–8, 1906
Soueges, R.: Les premieres divisions de l'oeuf et les différenciations du suspenseur chez leCapsella bursa-pastoris Moench. — Ann. Sci. nat. Bot.10: 1–28, 1919.
Vacin, E.F., Went, F.W.: Use of tomato juice in the asymbiotic germination of orchid seeds. — Bot. Gaz.111: 175–183, 1949.
Van Overbeek, J., Siu, R., Haagen-Smit, A.J.: Factors affecting the growth ofDatura embryosin vitro. — Amer. J. Bot.31: 219–224, 1944.
Wakizuka, T., Nakajima, T.: Development of proembryo in cultured ovules ofPetunia hybrida Vilm. — Jap. J. Breed.25: 161–167, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Monnier, M., Clippe, A. Effect of plant extracts on development ofCapsella embryos in ovules cultured invitro . Biol Plant 34, 31–38 (1992). https://doi.org/10.1007/BF02925787
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02925787