Abstract
If heterocontinuous flow-cultivation method was used to study the degradation of soluble carboxymethyl cellulose (CMC) in soil, neither the potential CM-cellulase activity of the soil nor the total degree of CMC mineralization significantly differed under aerobic condition and in a nitrogen atmosphere. In contrast, the end products of the enzymatic hydrolysis and their mutual proportions were different: under anaerobic conditions, the formation of reducing sugars was increased at the expense of CO2 production and organic acids were detectable in the extract. The composition of soil microflora also differed. Addition of ammonium ions affected the maximum CM-cellulase activity in the soil, the degree of substrate mineralization, the proportion of CO2 and reducing sugars that are formed and the concentration of the present soil microflora.
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The authors thank Dr. M. Balíkevá from theInstitute of Toxicology and Forensic Chemistry of the Faculty of Medicine, Charles University, Prague, for help with the analysis of organic acids.
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Mašková, H.P., Kunc, F. Microbial decomposition of carboxymethyl cellulose continuously added to the soil. Folia Microbiol 33, 474–481 (1988). https://doi.org/10.1007/BF02925774
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DOI: https://doi.org/10.1007/BF02925774