Skip to main content
SpringerLink
Log in

Movement of intrapancreatic calcium in dogs with experimental pancreatic lithiasis

With special reference to the process of pancreatic calculus formation

  • Published:
International journal of pancreatology Aims and scope Submit manuscript

Summary

This study was performed to clarify the movement of calcium in the pancreas of dogs with chronic pancreatitis, using scanning and transmission electron microscopes (SEM, TEM) equipped with X-ray elemental microanalyzers. Eleven adult mongrel dogs underwent incomplete ligation of the pancreatic duct. After the procedure, the levels of pancreatic enzymes in the serum did not change for 9 mo, but the endocrine function was reduced gradually. Of all dogs, 5 revealed pancreatic sclerosis, and 2 at 9 mo had calculi, 2–4 mm in size, in the small pancreatic duct. SEM examination revealed the intralobular fibrosis and irregularity of the pancreatic duct wall, and TEM examination demonstrated the amorphous or crystalloid substances and secreted granules in the acinar lumen or ductule. In elemental analysis spectra using SEM, a high calcium peak was seen in the pancreatic duct wall. In elemental analysis spectra using TEM, a high calcium peak was observed in the amorphous or crystalloid substances, and a high ratio of Ca/K was seen in these substances. Calcium was also detected in the secreted granules or microvilli These results suggest that there is a process of calculus formation based on the congregation of the intraductular substances containing a large quantity of calcium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sarles H. Chronic calcifying pancreatitis—chronic alcoholic pancreatitis.Gastroenterology 1974; 66: 604–616.

    PubMed  CAS  Google Scholar 

  2. Sarles H, Tiscornia O, Palasciano G, Brasca A, Hage G, Devaux MA, Gullo L. Effects of chronic intragastric ethanol administration on canine exocrine pancreatic secretion.Scand J Gastroenterol 1972; 8: 85–96.

    Google Scholar 

  3. Konishi K. Experimental pancreatic lithiasis.Jpn J Gastroenterol 1976; 73: 917–927.

    CAS  Google Scholar 

  4. Takayama T. Pathophysiological study of experimental pancreatolithiasis in the dog.Jpn J Gastroenterol 1979; 76: 1325–1336.

    CAS  Google Scholar 

  5. Katoh O. Experimental analysis on acidic mucopolysaccharides of canine pancreatic juice.Jpn J Gastroenterol 1983; 80: 863–871.

    CAS  Google Scholar 

  6. Harada H, Ueda O, Yasuoka M, Nakamura T, Hayashi T, Kobayashi T, Kimura I. Scanning electron-microscopic studies on protein plugs obtained from patients with chronic pancreatitis.Gastroenterol Jpn 1982; 17: 98–101.

    PubMed  CAS  Google Scholar 

  7. Harada H, Takeda M, Tanaka J, Miki H, Ochi K, Kimura I. The fine structure of pancreatic stones as shown by scanning electron microscopy and X-ray probe microanalyzer.Gastroenterol Jpn 1983; 18: 530–537.

    PubMed  CAS  Google Scholar 

  8. Pitchumoni CS, Viswanathan KV, GeeVarghese PJ, Banks PA. Ultrastructure and elemental composition of human pancreatic calculi.Pancreas 1987; 2: 152–158.

    Article  PubMed  CAS  Google Scholar 

  9. Harada H, Ueda O, Yasuoka M, Nakamura T, Kunichika K, Ikubo I, Kochi F, Shigetoshi K, Yabe H, Hanafusa E, Hayashi T, Takeda M, Mishima K, Kimura I, Tanaka T. Histochemical studies on protein plugs obtained by endoscopic retrograde catheterization of the papilla,Gastroenterol Jpn 1981; 16: 563–567.

    PubMed  CAS  Google Scholar 

  10. Yamaguchi A. Biochemical analysis of proteins in pancreatic calculi—with special reference to γ- carboxyglutamic acid-containing protein.Jpn J Gastroenterol 1983; 80: 1309–1317.

    CAS  Google Scholar 

  11. Heuser JE, Reese TS, Dennis MJ, Jan Y, Jan L, Evans L. Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release.J Cell Biology 1979; 81: 275–300.

    Article  CAS  Google Scholar 

  12. Kurihara T, Takehara T, Odashima S, Hirai K. Epoxy resin-embedded metal standards for quantitative X-ray microanalysis of biological materials.J Electron Microsc 1989; 38: 477–479.

    CAS  Google Scholar 

  13. Sakakibara A, Okumura N, Hayakawa T, Kanzaki M. Ultrastructural changes in the exocrine pancreas of experimental pancreatolithiasis in dogs.Am J Gastroenterol 1982; 77: 498–503.

    PubMed  CAS  Google Scholar 

  14. Kanno T. Advances in physiology of endocrine and exocrine pancreas.Igaku No Ayumi 1988; 144: 321–326.

    CAS  Google Scholar 

  15. Zimmerman MJ, Dreiling DA, Rosenberg IR, Janowitz HD. Secretion of calcium by the canine pancreas.Gastroenterology 1967; 52: 865–870.

    PubMed  CAS  Google Scholar 

  16. Goebell H, Baltzer G, Schlott KA, Bode CH. Parallel secretion of calcium and enzymes by the human pancreas.Digestion 1973; 8: 336–346.

    Article  PubMed  CAS  Google Scholar 

  17. Konishi K. Experimental pancreatic lithiasis.Jpn J Gastroenterol 1976; 73: 1519–1526.

    CAS  Google Scholar 

  18. Bank S, Marks IN, Vinik AI. Clinical and hormonal aspects of pancreatic diabetes.Am J Gastroenterol 1975; 64: 13–22.

    PubMed  CAS  Google Scholar 

  19. Homma T. Medical treatment of chronic pancreatitis.Igaku No Ayumi 1988; 144: 485–487.

    Google Scholar 

  20. Edmondson HA, Bullock WK, Mehl JW. Chronic pancreatitis and lithiasis, II. Pathology and pathogenesis of pancreatic lithiasis.Am J Path 1950; 26: 37–55.

    PubMed  CAS  Google Scholar 

  21. Naguro T, Iino A. Fine structure of the pancreas-scanning electron microscopic observation on the exocrine cells.Kan Tan Sui 1986; 13: 1022–1032.

    Google Scholar 

  22. Hosoi M, Nishimori T, Akisaka T, Imanishi I. Elemental analysis of calcifying hard tissues by analytical electron microscopy, II Ca/P ratios of human enamel crystals and dentin crystals.Jpn J Oral Biol 1982; 24: 216, 217.

    CAS  Google Scholar 

  23. Pitchumoni CS, Viswanathan KV. Trace element composition of pancreatic calculi (PC).Gastroenterology 1985; 88: 1540.

    Google Scholar 

  24. Schultz AC, Moore PB, GeeVarghese PJ, Pitchumoni CS. X-ray diffraction studies of pancreatic calculi associated with nutritional pancreatitis.Dig Dis Sci 1986; 31: 476–480.

    Article  PubMed  CAS  Google Scholar 

  25. Matsumoto M, Suzuki F, Ohi I, Ohashi M. Chronic pancreatitis, microelemental analysis of pancreatic calculus obtained from pancreatolithiasis. 1982-Yearly Report of Investigation and Research Group on Intractable Pancreatic Disease, Supported by a Grant-in Aid for Scientific Research, Ministry of Public Health and Welfare, Japan, Takeuchi T., ed., Tokyo, pp. 85–99, 1982.

  26. Estevenon JP, Sarles H, Figarella C. Lactoferrin in the duodenal juice of patients with chronic calcifying pancreatitis.Scand J Gastroent 1975; 10: 327–330.

    PubMed  CAS  Google Scholar 

  27. Kitagawa M, Hayakawa T, Kondo T, Shibata T, Sakai Y, Sobajima H, Ishiguro H, Tanikawa M, Nakae Y, Nimura Y, Kamiya J, Kondo S, Yasui A. Pancreatic stone predominantly composed of fatty acid calcium.Gastroenterology 1992; 102: 2151–2154.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Cancer Research, Kanazawa Medical University, 920-02, Uchinada-Machi, Kahoku-Gun, Ishikawa-Ken, Japan

    Yoshio Kinami

  2. Department of Surgery II, Kanazawa Medical University, Uchinada

    Yoshio Kinami, Masahiro Matsushita & Ichiro Kita

  3. Basic Science, Medical Research Institute, Kanazawa University, Kanazawa, Japan

    Takayuki Kurihara

  4. School of Medical Technique, Kanazawa University, Kanazawa, Japan

    Minoru Mizukarni

Authors
  1. Yoshio Kinami
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Masahiro Matsushita
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ichiro Kita
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takayuki Kurihara
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Minoru Mizukarni
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kinami, Y., Matsushita, M., Kita, I. et al. Movement of intrapancreatic calcium in dogs with experimental pancreatic lithiasis. Int J Pancreatol 13, 221–229 (1993). https://doi.org/10.1007/BF02924444

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02924444

Key Words

Search

Navigation