Summary
The activity of phospholipase A2 (PA2) was measured in the plasma of rats after the induction of acute pancreatitis. Buffered sodium taurocholate (3.75% w/v), injected into the pancreatico-biliary duct, was used to induce acute pancreatitis. Blood was taken at 1-h intervals for the measurement of phospholipase A2, glucose, lipase, and arterial gases. Plasma lipase was markedly elevated in the test groups, indicating acute pancreatitis, but no significant difference in PA2 activity was seen between the controls and the test group. However, in four animals the PA2 activity was elevated and in all cases this was accompanied by a low arterial pO2. This supports the theoretical role of PA2 in the pathogenesis of the respiratory complications observed in severe acute pancreatitis.
Similar content being viewed by others
References
Nevalainen TJ. The role of phospholipase A in acute pancreatitis. Scand. J. Gastroent. 1980; 15: 641–650.
Schmidt H, Creutzfeld W. The possible role of phospholipase A in the pathogenesis of acute pancreatitis. Scand. J. Gastroent. 1969; 4: 39–48.
Aho HJ, Nevelainen TJ, Lindberg RLP, Aho AJ. Experimental pancreatitis in the rat. The role of phospholipase A in sodium taurocholate-induced acute haemorrhagic pancreatitis. Scand. J. Gastroent. 1980; 15: 1027–1031.
Zeive L, Vogel WC. Measurement of lecithinase-A in serum and other body fluids. J. Lab. Clin. Med. 1961; 57: 586–599.
Morgan AP, Jenny ME, Hassler H. Phospholipids, acute pancreatitis and the lungs. Ann. Surg. 1968; 167: 329–335.
Dougall AM, Brennan MM, Wong CY, et al. Effects of experimental haemorrhagic pancreatitis on respiratory function and pulmonary phospholipids. Surg. Forum 1977; 28: 1189–191.
McIver AG, Metcalf IL, Ponmayer F, Harding PGR, Pasi RB. Alteration of surfactant in experimental haemorrhagic pancreatitis. J. Surg. Res. 1977; 23: 311.
Reinitz ER, Motoyama E, Smith GJW, Kerstein MD. Pulmonary sequelae of experimental pancreatitis. J. Surg. Res. 1977; 22: 566–579.
Heinkel K. Die ratte als Versuchstier in der experimentellen pankreasdiagnostik. II. Mitteilung. Die erzeugung einer akuten hamorrhagischen pankreatitis durch injektion von gallensaure in der ductus pancreaticus. Klin. Wschr. 1953; 31: 815.
Aho HJ, Kosensalo SML, Nevalainen TL. Experimental pancreatitis in the rat. Sodium taurocholate-induce acute haemorrhagic pancreatitis. Scans. J. Gastroent. 1980; 15: 411–416.
Aho HJ, Suonpaa K, Ahola RA, Nevalainen TJ. Experimental pancreatitis in the rat. Ductal factors in sodium taurocholate induced acute pancreatitis. Exp. Path. 1984; 25: 73–79.
Bird NC. A new method for measuring pancreatic phospholipase A2. Clin. Chim. Acta 1982; 125: 359–365.
Gjone W, Ofstade E, Marton PF, Amundsen E. Phospholipase activity in pancreatic exudate in experimental pancreatitis. Scand. J. Gastroent. 1967; 2: 181–185.
Etienne J, Gruber A, Polonowski J. Activite phospholipasique A2 du serum de rat. Association de deux proteines. Biochim. Biophys. Acta 1980; 619: 693–698.
Singh C, Ramesh V, Anjaneyulu KA. Comparative study of serum phospholipase A2 in man and animals—some biochemical and physiological implications. Indian J. Exp. Biol. 1979; 17 (1): 50–57.
Kaplan-Harris L, Wiess J, Mooney C, Beckerdite-Quagliata S, Elsbach P. The action of human and rabbit serum phospholipase A2 onEscherichia coli phospholipids. J. Lipid Res. 1980; 21: 617–624.
Etienne J, Gruber A, Polonowski, J. Phosphatidylethanolamine phospholipase A2 du serum de rat. Bull. Soc. Chim. Biol. 1967; 49: 1751–1754.
Till GO, Johnsson KJ, Kunkel R, Ward PA, Intravascular activation of complement and acute lung injury. J. Clin. Invest. 1982; 69: 1126.
Spagnuolo PJ, Ellner JJ, Hassid A, Dunn MJ: Thromboxane A2 mediates augmented polymorphonuclear leukocyte adhesiveness. J. Clin. Invest. 1980; 66: 406.
Barie PS, Tahamont MV, Malik AB. Prevention of increased pulmonary vascular permeability after pancreatitis by granulocyte depletion in sheep. Am. Rev. Respir. Dis. 1982; 126: 904.
Till GO, Beauchamp C, Mdnapace D et al. Oxygen radical-dependant lung damage following thermal injury of rat skin. J. Trauma 1983; 23: 269.
Ward PA, Till GO, Kunkel R, Beauchamp C. Evidence for the role of hydroxyl radical in neutrophil-dependent tissue injury. J. Clin. Invest. 1983; 72: 789.
Schraufstatter, IU, Revak SD, Cochrane CG. Proteases and oxidants in experimental pulmonary inflammatory injury. J. Clin. Invest. 1984; 73: 1175.
Eskola JU, Nevalainen TJ, Lovgren TNE. Time resolved fluoroimmunoassay of human pancreatic phospholipase A2. Clin. Chem. 1983; 29 (10): 1777–1780.
Nishijima J, Okamoto M, Ogawa M, Kosaki G, Yamano T. Purification and characterization of human pancreatic phospholipase A2 and development of a radioimmunoassay. J. Biochem. 1983; 94: 137–147.
Nevalainen TJ, Eskola JU, Aho AJ, Havia T, Lovgren TNE, Nanto V. Immunoreactive phospholipase A2 in serum in acute pancreatitis and pancreatic cancer. Clin. Chem. 1983; 70: 696.
Bird NC, Johnson AG. Serum phospholipase A2 levels in acute pancreatitis. Brit. J. Surg. 1983; 70: 696.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bird, N.C., Peng, S.Y., Goodman, A.J. et al. Phospholipase A2 activity in tauroeholate-induced acute pancreatitis in the rat model. Int J Pancreatol 4, 3–11 (1989). https://doi.org/10.1007/BF02924143
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02924143