Abstract
The growth of callus tissue cultures and the infectivity of twenty fiveSolanum laciniatum Ait. plants and of sixteenNicotiana tabacum L. cv. Samsun plants were investigated. The plants were obtained from callus tissue cultures derived from stem pieces of the respective plants infected with a mycoplasma-like organism (MLO) evoking potato witches’ broom. The tissues were cultivated on synthetic nutrient medium with kinetin and IAA. Allde novo obtainedS. laciniatum plants were healthy. On the contrary twelve of the sixteen reconstituted tobacco plants showed MLO presence.
Summarizing these and previous results, the authors suppose that the most important factor influencing MLO persistence in callus tissues cultivated on the applied nutrient medium may be the callus growth rate and the organogenesis set. Both these conditions are determined by the metabolism of the investigated plant species.
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References
Gautheret, R. J.: Factors affecting differentiation of plant tissues grownin vitro. - In: Cell Differentiation and Morphogenesis. International Lecture Course. Pp. 55-96. Wageningen 1966.
Jacoli, G. G.: Translocation of mycoplasma-like bodies through sieve pores in plant tissue cultures infected with aster yellows. -Can. J. Bot.52: 2085–2088, 1974.
Jacoli, G. G., Ronald, W. P.: Electron microscopic studies of plant tissue cultures infected with the aster yellows disease. - J. Ultrastruct. Res.46: 32–42, 1974.
Petbů, B., Limberk, J., Ulryohová, M., Brčák, J.: Growth and infectivity of callus cultures of tomato plants infected with a mycoplasma disease - potato witches’ broom. - Biol. Plant.13: 391–395, 1971.
Petbů, E., Ulrychová, M.: Persistence and spread of mycoplasma in axenic callus tissue cultures of tobacco (Nicotiana glauca Grah.) in the presence of kinetin and IAA in nutrient medium. - Biol. Plant.17: 352–356, 1975.
Petrů, E., Ulrychová, M.: [Callose formation in the sieve tubes of a callus tissue culture of tobacco plants (Nicotiana glauca Grah.) infected with mycoplasma.] In Czech. - Kolokvium o rastlinných explantátových kulturách. Stupy pri Pezinku 2.–5. decembra 1974. Pp. 177–189. Universita Komenského, Bratislava 1976.
Petrů, E.(Ulrychová M.: [The problem of mycoplasma persistence in callus tissues derived from some host plants of the familySolanaceae.] In Czech. - In:Novák, P. J.: (ed.): Využiti kultur rostlinných explantátů ve šlechtění. Sbor. mezinár. symp. 6. -11. září 1976, Olomouc ČSSR. Pp. 509–514. ČSAV, Ústav exp. Bot., Praha 1977.
Petrů, E.,Ulrychová, M.,Limberk, J.: [Growth and properties of calli isolated from plants infected with mycoplasma duringin vitro cultivation.] In Czech. - Proo. IVth Conf. Plant Protection. Pp. 73-79, Bratislava 1972.
Ulrychová, M., Jokeš, M.: Mycoplasma-like bodies inSolanum laciniatum plants infected with potato witches’ broom disease. - Biol. Plant.19: 248–252, 1977.
Ulrychová, M., Limberk, J.: Some factors affecting the spread of mycoplasma in plants. - Biol. Plant.14: 233–240, 1972.
Ulrychová, M., Petrš, E.: Elimination of mycoplasma in tobacco callus tissues (Nicotiana glauca Gbah.) culturedin vitro in the presence of 2,4-D in nutrient medium. - Biol. Plant.17: 103–108, 1975.
Valenta, V.: Potato witches’ broom virus in Czechoslovakia. - Proc. 3rd Conf. Potato Diseases. Pp. 246-250, Wageningen 1958.
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Petrů, E., Ulrychová, M. Behaviour of mlo evoking potato witches’ broom in callus tissue culture ofSolatium laciniatutn ait. andNicotiana tabacum L. cv. samsun. Biol Plant 20, 383–386 (1978). https://doi.org/10.1007/BF02923334
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DOI: https://doi.org/10.1007/BF02923334