Skip to main content
Log in

Selective shift in the metabolic pathways of excised tomato cotyledons in response to mannitol induced water stress

  • Published:
Biologia Plantarum

Abstract

Excised tomato cotyledons subjected to mannitol induced water stress had more total sugars than normal cotyledons during injury but less total sugars than normal cotyledons in the adaptive phase. During water stress injury, protein and UNA synthesis were reduced, activities of beta-fructofuranosidase and phosphofructokinase were reduced but activities of proteases, ribonuclease, hexokinase and glucose-6-phosphate dehydrogenase were increased. Additions of kinetin reversed the water stress effects on enzyme activities. It is concluded that during water stress injury, there was a fundamental reduction in cytokinin activities leading to the selective shift in the enzyme populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Barnett, N. M., Naylor, A. W.: Amino-acid and protein metabolism in Bermuda grass during water stress. - Plant Physiol.41: 1222–1230, 1966.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Boyer, J. S.: Differing sensitivity of photosynthesis to low leaf water potentials in corn and soybean. - Plant Physiol.46: 236–239, 1970.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Cherry, J. H.: Regulation of invertase in washed sugar beet tissues. - In:Wightmann, P., Setterfield, G. (ed.): Biochemistry and Physiology of Plant Growth Substances. Pp. 417 to 432. The Runge Press, Ottawa 1967.

    Google Scholar 

  • Chrispeel, M. J., Varner, J. E.: Gibberellic acid enhanced synthesis and release of alpha-amylase and ribonuclease by isolated aleurone layer of barley. - Plant Physiol.42: 1008 to 1016, 1967.

    Google Scholar 

  • Dubios, M., Gilles, K. A., Hamilton, J. K., Rebes, P. A., Smith, F.: Colorimetric method for determination of sugars and related substances. - Anal. Chem.28: 350–356, 1956.

    Article  Google Scholar 

  • Jacobson, J. V., Varner, J. E.: Gibberellic acid induced synthesis of protease by isolated aleurone layers of barley. - Plant Physiol.42: 1596–1600, 1967.

    Article  Google Scholar 

  • Joshi, M. D., Jaganathan, V.: Hexokinase. - In:Colowick, S. P., Kaplan, N. O. (ed.): Methods in Enzymology. Vol. 9. Pp. 371–375. Acad. Press, New York-London 1966.

    Google Scholar 

  • Kenten, R. H.: Gasometric analysis in plant investigations. - In:Peach, K., Traoey, M. V. (ed.): Modern Methods of Plant Analysis. Vol. 1. P. 415, 1956.

    Google Scholar 

  • Langdon, R. G.: Glucose-6-phosphate dehydrogenase from erythrocytes. - In:Colowick, S. P., Kaplan, N. O. (ed.): Methods in Enzymology. Vol. 9. Pp. 126–131. Acad. Press, New York- London 1966.

    Google Scholar 

  • Lee, Y. P., Takaheshi, T.: An improved colorimetric determination of amino-acids with the use of ninhydrin. -Anal. Biochem.14: 71–77, 1966.

    Article  CAS  Google Scholar 

  • Levitt, J.: The Hardiness of Plants. - Acad. Press, New York 1956.

    Google Scholar 

  • Ling, K. H., Paetkau, V., Marcus, F., Lardy, H. A.: Phosphofructokinase. I. Skeletal muscle.- In:Colowick, S. P., Kaplan, N. O. (ed.): Methods in Enzymology. Vol. 9. Pp. 425–429. Acad. Press, New York-London 1966.

    Google Scholar 

  • McDonald, M. R.: Ribonuclease. - In:Colowick, S. P., Kaplan, N. O. (ed.): Methods in Enzymology. Vol. 2. Pp. 427–436. Acad. Press, New York-London 1955.

    Google Scholar 

  • Nelson, N.: A photometric adaptation of the Somogyi method for determination of glucose. - J. biol. Chem.153: 375–380, 1944.

    CAS  Google Scholar 

  • Ong, H. T.: Roles of hormones in the responses of excised tomato cotyledons to mannitol induced water stress. - Biol. Plant.20: 318–323, 1978.

    Article  Google Scholar 

  • Plaut, Z., Ordin, L.: The effects of moisture tension and nitrogen supply on cell wall metabolism of sunflower leaves. - Physiol. Plant.17: 279–286, 1964.

    Article  CAS  Google Scholar 

  • Schmidt, G., Thannhauser, S. J.: A method for the determination of DNA, RNA and phosphoprotein in animal tissues.- J. biol. Chem.161: 83–89, 1945.

    CAS  PubMed  Google Scholar 

  • Woodhams, D. H., Kozlowski, T. T.: Effects of soil moisture stress on carbohydrates development and growth in plants. - Am. J. Bot.41: 316–328, 1954.

    Article  CAS  Google Scholar 

  • Zholkevitch, V. N., Korestkaya, T. F.: [Metabolism of pumpkin roots during soil drought.] In Russ. - Fiziol. Rast.6: 690–700, 1959.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tatt, O.H. Selective shift in the metabolic pathways of excised tomato cotyledons in response to mannitol induced water stress. Biol Plant 20, 324–329 (1978). https://doi.org/10.1007/BF02923322

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02923322

Keywords

Navigation