Abstract
Long-term callus cultures of two barley cultivars have been investigated at the cellular level. One slowly growing, root forming callus of the barley cv. Bulgarische Nackte consists of cells the majority of which contain the diploid chromosome number. Contrarily, a fast growing callus of the cv. Elgina having no regeneration potency at all, is highly polyploid and includes more than two thirds of aneuploid cells. Tn this callus strain, a great number of multinucleate cells has been found. Some problems are discussed with respect to the relationships between growth rate, karyological stability and regeneration capacity in long-term callus cultures of cereals.
Similar content being viewed by others
References
D’Amato, F.: Endopolyploidy as a factor in plant tissue development. - Caryologia17: 41–52, 1964.
Asami, H., Shimada, T., Inomata, N., Okamoto. M.: Chromosome constitution in cultured callus cells from four aneuploid lines of the homoeologous group 5 ofTriticum aestivum. - Jap. J. Genet.50: 283–289, 1975.
Bayliss, M. W.: Origin of chromosome number variation in cultured plant cells. - Nature146: 529–530, 1973.
Bennett, M. D., Finch, R. A.: Mitotic cycle time of root meristeme cells ofHordeum vulgare. - Caryologia25: 439–448, 1972.
Böök, J. A.: Cytological studies in Triton. - Hereditas31: 177–220, 1945.
Gcpta, S., Gadgil, V. N.: Multinucleate cells in normal and crown gall tumor tissue cultures. - Ind. J. exp. Biol.11: 306–309, 1973.
Heinz, D. J., Mee, G. W. P., Nickell, L. G.: Chromosome numbers of someSaccharum species hybrids and their cell suspension cultures. - Amer. J. Bot.56: 450–456, 1969.
Kallak, H., Yarvekylg, L.: Nuclear behaviour in callus cells: morphology and division. - Biol. Plant.19: 48–52, 1977.
Kao, K. N., Miller, R. A., Gamborg, O. L., Harvey, B. L.: Variations in chromosome number and structure in plant cells grown in suspension cultures. - Can. J. Genet. Cytol.12: 297–301, 1970.
KüNZEl, G.: Über die Beeinflussbarkeit der aberrationsauslösenden Wirkung von N-Nitroso- -N-Mothylharnstoff auf die Chromosomen bei Gerste. - Kulturpflanze16: 151–172, 1968.
Mahlberg, P. G., Turner, F. R., Walkinshaw, Ch., Venketeswaran, S., Mehtrotra, B.: Observations on incomplete cytokinesis in callus cells. - Bot. Gaz.136: 189–195, 1975.
Melchers, G., Bergmann, L.: Untersuchungen an Kulturen von haploiden Geweben vonA ntirrhinum majus. - Ber. deut. bot. Ges.71: 459–473, 1958.
Norstog, K., Wall, W. E., Howland, G. P.: Cytological characteristics of ten-year-old rye-grass endosperm cultures. - Bot. Gaz.130: 83–86, 1969.
Reinert, J., Torrey, J. G.: Über die Kultur von Geweben ausHaplopappus gracilis. -Naturwissenschaften48: 132–133, 1961.
Rieger, R.: Die Genommutationen (Ploidiemutationen). - VEB Gustav-Fischer-Verlag, Jena 1963.
Schetjnert, E.-U., Shamina, Z. B., Koblitz, H.: Studies on barley calluses culturedin vitro. I. Establishment, maintenance and growth of two different tissue strains. - Plant Sci. Lett.10: 313–318, 1977.
Shamina, Z. B.: [Genetical investigations onin vitro cultivated plant cells]. In Russ. - Sec. All-Union Conf. on Plant and Cell Culture. Kiev 1975.
Shamina, Z. B.,Scheunert, E.-U.,Koblitz, H.: Studies on barley calluses culturedin vitro. II. Cytological and morphological features of barley callus tissues culturedin vitro. - Plant Sci. Lett, in press, 1978.
Singh, B. D., Harvey, B. L.: Does 2,4-D induce mitotic irregularities in plant tissue cultures? - Experientia31: 785–787, 1975.
Smith, L.: Cytology and genetics of barley. - Bot. Rev.17: 1–51, 133–202, 285–355, 1951.
Torrey, J. G.: Kinetin as a trigger for mitosis in nature endomitotie plant cells. - Exp. Cell Res.23: 281–299, 1961.
Author information
Authors and Affiliations
Additional information
Studies on Barley Calluses Culturedin vitro: Part III.
Rights and permissions
About this article
Cite this article
Scheunert, E.U., Shamina, Z.B. & Koblitz, H. Karyological features of barley callus tissues culturedin vitro . Biol Plant 20, 305–308 (1978). https://doi.org/10.1007/BF02923317
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02923317