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Autoradiographic detection of uptake of bacterial3H-DNA label by barley seeds and isolated barley embryos

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Biologia Plantarum

Abstract

Barley seeds and extirpated embryos were cultivated in3H-DNA fromBacillus subtilis. Control group of embryos was cultivated in3H-thymidine. Uptake of the label from3H-DNA was observed after both methods of application, to halves of seeds and to embryos, and the label was localized almost exclusively in some of the nuclei of meristematic cells.

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Flemingovo nám. 2, 160 00, Praha 6, Czechoslovakia

    M. Ondřej

  2. Institute of Microbiology, Czechoslovak Academy of Sciences, BudĚjovická 1083, 142 20, Praha-Krč, Czechoslovakia

    P. Tichý

Authors
  1. M. Ondřej
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  2. P. Tichý
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Ondřej, M., Tichý, P. Autoradiographic detection of uptake of bacterial3H-DNA label by barley seeds and isolated barley embryos. Biol Plant 20, 67–69 (1978). https://doi.org/10.1007/BF02922932

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  • DOI: https://doi.org/10.1007/BF02922932

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