Abstract
The endonuclease activity specific for apurinic sites in DNA was detected in barley embryos. The enzyme was partially purified. It reveals high activity on partially depurinated DNA but low or nil activity on intact and alkylated DNA. The method used for the detection of enzyme activity was based on the changes in the sedimentation velocity of substrate DNA in neutral sucrose gradients with 80 % formamide.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gaudin, D., Yielding, K. L.: The use of formamide gradients to distinguish between alkali labile regions and single-strand breaks in DNA. — Biochem. biophys. Res. Commun.47: 1396 to 1401, 1972.
Kuhklein, U., Penhoet, B. E., Linn, S.: An altered apurinic DNA endonuclease activity in group A and group DXeroderma pigmentosum fibroblasts. — Proc. nat. Acad. Sci. USA73: 1169–1173, 1976.
Ljungquist, S., Lindahl, T.: A mammalian endonuclease specific for apurinic sites in doublestranded deoxyribonucleic acid. — J. biol. Chem.249: 1530–1535, 1974.
Mabmur, J.: A procedure for isolation of deoxyribonucleic acid from microorganismus. — J. mol. Biol.3: 208–218, 1961.
Melgar, E., Goldthwait, D. A.: Deoxyribonucleic acid nucleases. I. The use of a new method to observe the kinetics of deoxyribonucleic acid degradation by deoxyribonuclease I, deoxyribonuolease II, andEseherichia coli endonuclease I. — J. biol. Chem.243: 4401–4408, 1968.
Paquette,Y., Crine, P., Vebly, W. G.: Properties of the endonuclease for depurinated DNA fromEtcheriehia coli. — Canad. J. Biochem.50: 1199–1209, 1972.
ŠvachulovÁ, J.,Šatava, J.,VelemÍnskÝ, J.: A barley endonuclease specific for apurinic DNA: Isolation and partial characterisation. — Europ. J. Biochem. (Submitted for publication.)
Thibodeau, L., Verly, W. G.: Endonuclease for apurinic sites in plants. — FEBS Lett.69: 183 to 185, 1976.
Tomilin, N. V.: [On the presence of an endonuclease acting on UV — irradiated and depurinated DNA inMicrococcus lysodeikticus cells]. In Russ.— Mol. Biol.8: 557–568, 1974.
Ts’o, P. O. P., Helmkamp, K., Sander, CH.: Secondary structure of nucleic acids in organic solvents. II. Optical properties of nucleotides and nucleic acids. — Biochim. biophys. Acts55: 584–600, 1962.
VelemÍnskÝ, J., ZadbaŽil, S., Gichneb, T.: Repair of single-strand breaks in DNA and recovery of induced mutagenic effects during the storage of ethyl-methane-sulphonate-treated barley seeds. — Mutation Res.14: 259–261, 1972.
VelemÍnskÝ, J., ZadraŽil, S., PokornÝ, V., Gichner, T., ŠvachulovÁ, J.: Repair of singlestrand breaks and fate of N-7-methylquanine in DNA during the recovery from genetical damage induced by N-methyl-N-nitrosourea in barley seeds. — Mutation Res.17: 49–58, 1973.
VelemÍnskÝ, J.,ZadraŽil, S.,PokobnÝ, V.,Gichner, T.: DNA repair synthesis stimulated by mutagenic N-Methyl-N-nitrosourea in barley seeds and free embryos. — Mutation Res. (in press).
Verly, W. G., Paquette, Y., Thibodeau, L.: Nuclease for DNA apurinic sites may be involved in the maintenance of DNA in normal cells. — Nature244: 67–69, 1973.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vblemínský, J., Švachulová, J. & Šatava, J. Isolation from barley embryo of endonuclease specific for apurinic sites in DNA. Biol Plant 19, 346–352 (1977). https://doi.org/10.1007/BF02922729
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02922729