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Mechanism and improvement of complex formation between LPS and polymyxin B that is immobilized on an ion exchanger

Convenient immobilization procedures for spacer and polymyxin B

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Abstract

Polymyxin B was immobilized on the ion exchanger, Amberlite IRC-50. The modified support was used as an adsorbent for lipopolysaccharide ofE. coli from a phosphate buffer at pH = 7.0 and T = 20°C. The insertion of a spacer, 6-amino hexanoic acid, improved the adsorption capacity of the modified carrier significantly. Upon partial acetylation (25%) of primary amino groups of immobilized Polymyxin B, the adsorption capacity of the support was halved. From our results, it is concluded that the complex formation between lipopolysaccharide and immobilized Polymyxin B is based on both lipophilic, as well as electrostatic, interactions. Convenient procedures for the immobilization of the ligands and the characterization of the ion exchanger are described.

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Authors and Affiliations

  1. Departments of Organic Chemistry, Eindhoven University of Technology, Den Dolech 2, PO Box 513, 5600 MB, Eindhoven, The Netherlands

    M. A. F. A. Boons, L. A. Ae. Sluyterman & H. M. Buck

  2. Instrumental Analysis, Eindhoven University of Technology, Den Dolech 2, PO Box 513, 5600 MB, Eindhoven, The Netherlands

    M. L. P. M. Verhoeven

Authors
  1. M. A. F. A. Boons
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  2. M. L. P. M. Verhoeven
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  3. L. A. Ae. Sluyterman
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  4. H. M. Buck
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Boons, M.A.F.A., Verhoeven, M.L.P.M., Sluyterman, L.A.A. et al. Mechanism and improvement of complex formation between LPS and polymyxin B that is immobilized on an ion exchanger. Appl Biochem Biotechnol 22, 95–107 (1989). https://doi.org/10.1007/BF02922699

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  • DOI: https://doi.org/10.1007/BF02922699

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