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Biological production of acetaldehyde from ethanol using non-growingPichia pastoris whole cells

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Abstract

Acetaldehyde has been produced biologically using whole-cellPichia pastoris in a semibatch fermentor. Ethanol and air were fed continuously, and the product, acetaldehyde, was removed by the air stream. Operation of the reactor exceeded 100 h, maintaining high alcohol oxidase activity. Low cell-mass concentration (9.9 g/L) minimized product inhibition. Ethanol concentration in the broth, oxygen concentration in the air, and pH were evaluated for their effects on the fermentation process.

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Abbreviations

Ca, Ce :

acetaldehyde and ethanol conc. in liquid phase (mol/L).

Cai, Cei :

initial acetaldehyde and ethanol conc. (mol/L).

Ca *, Ce * :

acetaldehyde and ethanol cone, in gas phase (mol/L).

Ca, in:

input acetaldehyde conc. (mol/L).

F :

volumetric gas flow rate (L/h).

R a :

rate equation (mol/L/h).

t :

time (h).

V 1, Vg :

liquid and vapor phase volume (L).

Vo :

reactor total volume (Vg+V1) (L).

(dC a /dt)strp, (dCe / dt)strp :

the stripping rate of acetaldehyde and ethanol (mol/L/h).

References

  1. Byrne, B. and Shermen, G. (1984),Food Technol. 57–61.

  2. Windholz, M. (ed.) (1983),The Merck Index, 10th Ed., Merck, Rahway, NJ, pp. 6.

    Google Scholar 

  3. Bromann, C. and Sahn, H. (1978),Arch. Microbiol. 117, 67.

    Article  Google Scholar 

  4. Sahm, H. and Wagner, F. (1973),Eur. J. Biochem. 36, 250–256.

    Article  CAS  Google Scholar 

  5. Hopkins, T. R. (1985), US Patent 4, 540, 668.

  6. Hoiberg, D. A., Hatfield, G. W., and Moyed, H. S., PCT (World Intellectual Property Organization) W087/04725 (1987).

  7. Guilbault, G. G. and Kauffmann, J. M. (1987),Biotechnol. Biochem. 9, 95–113.

    CAS  Google Scholar 

  8. Barzana, E., Klibanov, A. M., and Karel, M. (1987),Appl. Biochem. Biotechnol. 15, 25–34.

    Article  CAS  Google Scholar 

  9. Boeiu, G. G., Dordick, J. S., and Klibanov, A. M. (1986),Biotechnol. 4, 997–999.

    Article  Google Scholar 

  10. Duff, S. J. B. and Murray, W. D. (1988),Biotechnol. Bioeng. 31, 44–49.

    Article  CAS  Google Scholar 

  11. Kierstan, M. (1982),Biotechnol. Bioeng. 24, 2275–2277.

    Article  CAS  Google Scholar 

  12. Duff, S. J. B. and Murray, D. (1988),Biotechnol. Bioeng. 31, 790–795.

    Article  CAS  Google Scholar 

  13. Couderc, R. and Baratt, J. (1980),Biotechnol. Bioeng. 22, 1155–1173.

    Article  CAS  Google Scholar 

  14. Tard, Y. (1985),Biotechnol. Gen. Eng. Rev. 3, 111–135.

    Google Scholar 

  15. Klibanov, A. M. (1983),Adv. Appl. Microbiol. 29, 1–28.

    Article  CAS  Google Scholar 

  16. Raymond, W. R. (1984),US Patent 4,481,292.

  17. Couderc, R. and Baratti, J. (1980),Agr. Biol. Chem. 44, 2279–2289.

    CAS  Google Scholar 

  18. Veenhuis, M.; Van Dijken, J. P., and Harder, W. (1983),Adv. in Microbiol. Phys.,24, 1–82.

    Article  CAS  Google Scholar 

  19. Wecker, M. S. A. and Zall, R. R. (1987),Appl. Environ. Microbiol. 53, 2815–2820.

    CAS  Google Scholar 

  20. Bormann, C. and Sahm, H. (1978),Arch. Microbiol. 117, 67.

    Article  CAS  Google Scholar 

  21. Armstrong, D. W., Martin, S. M., and Yamazaki, Hiroshi (1984),Biotechnol. Lett. 6, 183–188.

    Article  CAS  Google Scholar 

  22. Bailey, J. E. and Ollis, D. F. (1986),Biochemical Engineering Fundamentals, 2nd Ed., McGraw-Hill, New York.

    Google Scholar 

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Authors and Affiliations

  1. School of Chemical Engineering, Oklahoma State University, Stillwater, OK

    Heien-Kun Chiang & Gary L. Foutch

  2. Research and Development Center, Phillips Petroleum Company, Bartlesville, OK

    Wayne W. Fish

Authors
  1. Heien-Kun Chiang
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  2. Gary L. Foutch
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  3. Wayne W. Fish
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Chiang, HK., Foutch, G.L. & Fish, W.W. Biological production of acetaldehyde from ethanol using non-growingPichia pastoris whole cells. Appl Biochem Biotechnol 28, 513–525 (1991). https://doi.org/10.1007/BF02922630

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  • DOI: https://doi.org/10.1007/BF02922630

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